ArticleNature communications2018
Combinatorial hydrogels with biochemical gradients for screening 3D cellular microenvironments.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
What it found
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Who cites it
101 citing papers in PubMed.
- Surviving the Nucleus Pulposus Desert: Next-Generation Strategies for Intervertebral Disc Cell Therapy.JOR spine · 2026Article
- Programmable continuous gradient bioprinting for engineering spatially heterogeneous microenvironments.Materials today. Bio · 2026Article
- Integrating microchannels and flows into 3D printable granular hydrogel matrices.Lab on a chip · 2026Article
- The Degeneration Paradox: Severely Degenerated Cervical Nucleus Pulposus Cells Display Enhanced Mechanoplasticity Under Moderate Cyclic Tensile Strain.Biomolecules · 2026Article
- Functionally Graded Surfaces and Materials: From Fabrication to Biomedical Applications.Chemical reviews · 2026Review
- Programmable acoustofluidic engineering for creating gradient biomaterials.Science advances · 2025Article
- Effects of Stiffness and Degradability on Cardiac Fibroblast Contractility and Extracellular Matrix Secretion in Three-Dimensional Hydrogel Scaffolds.ACS biomaterials science & engineering · 2025Article
- Curved Magnetic Hydrogels for Understanding Cancer Initiation.ACS applied materials & interfaces · 2025Article
- Review
- Extracellular Peptide-Ligand Dimerization Actuator Receptor Design for Reversible and Spatially Dosed 3D Cell-Material Communication.ACS synthetic biology · 2025Article
- Cell-Instructive Biomaterials with Native-Like Biochemical Complexity.Annual review of biomedical engineering · 2025Review
- Mesenchymal Stromal Cell Chondrogenic Differentiation Induced by Continuous Stiffness Gradient in Photocrosslinkable Hydrogels.Journal of biomedical materials research. Part A · 2025Article
- Combinatorial strategy for engineering cartilage and bone microtissues using microfluidic cell-laden microgels.Biofabrication · 2025Article
- Recent regulatory developments in EU Medical Device Regulation and their impact on biomaterials translation.Bioengineering & translational medicine · 2025Review
- Chemically defined and dynamic click hydrogels support hair cell differentiation in human inner ear organoids.Stem cell reports · 2025Article
- Light-based fabrication and 4D customization of hydrogel biomaterials.Nature reviews bioengineering · 2025Article
- Photocrosslinkable Biomaterials for 3D Bioprinting: Mechanisms, Recent Advances, and Future Prospects.International journal of molecular sciences · 2024Review
- Zonal patterning of extracellular matrix and stromal cell populations along a perfusable cellular microchannel.Lab on a chip · 2024Article
- 3D Bioprinting Using Universal Fugitive Network Bioinks.ACS applied bio materials · 2024Article
- Gradient Functionalization of Poly(lactic acid)-Based Materials with Polylysine for Spatially Controlled Cell Adhesion.Polymers · 2024Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
3D microenvironmental parameters control cell behavior, but can be challenging to investigate over a wide range of conditions. Here, a combinatorial hydrogel platform is developed that uses light-mediated thiol-norbornene chemistry to encapsulate cells within hydrogels with biochemical gradients made by spatially varied light exposure. Specifically, mesenchymal stem cells are photoencapsulated in norbornene-modified hyaluronic acid hydrogels functionalized with gradients (0-5 mM) of peptides that mimic cell-cell or cell-matrix interactions, either as single or orthogonal gradients. Chondrogenesis varied spatially in these hydrogels based on the local biochemical formulation, as indicated by Sox9 and aggrecan expression levels. From 100 combinations investigated, discrete hydrogels are formulated and early gene expression and long-term cartilage-specific matrix production are assayed and found to be consistent with screening predictions. This platform is a scalable, high-throughput technique that enables the screening of the effects of multiple biochemical signals on 3D cell behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.