ArticleParticle and fibre toxicology2018
Differences in MWCNT- and SWCNT-induced DNA methylation alterations in association with the nuclear deposition.
Article in Particle and fibre toxicology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 69 citations in OpenAlex.
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- The Effect of Nanomaterials on DNA Methylation: A Review.Nanomaterials (Basel, Switzerland) · 2023Review
- Hepatic Glucose Metabolism Disorder Induced by Adipose Tissue-Derived miR-548ag via DPP4 Upregulation.International journal of molecular sciences · 2023Article
- Carbon nanotube nanocomposite scaffolds: advances in fabrication and applications for tissue regeneration and cancer therapy.Frontiers in bioengineering and biotechnology · 2023Review
- Applying the exposome concept to working life health: The EU EPHOR project.Environmental epidemiology (Philadelphia, Pa.) · 2022Article
- Quercetin-Ameliorated, Multi-Walled Carbon Nanotubes-Induced Immunotoxic, Inflammatory, and Oxidative Effects in Mice.Molecules (Basel, Switzerland) · 2022Article
- Epigenetic Mechanisms in Understanding Nanomaterial-Induced Toxicity.Advances in experimental medicine and biology · 2022Article
- A success targeted nano delivery to lung cancer cells with multi-walled carbon nanotubes conjugated to bromocriptine.Scientific reports · 2021Article
- Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years.International journal of molecular sciences · 2021Article
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- Femtosecond pulsed laser microscopy: a new tool to assess the in vitro delivered dose of carbon nanotubes in cell culture experiments.Particle and fibre toxicology · 2021Article
- Epigenetic Effects of Nanomaterials and Nanoparticles.Journal of nanobiotechnology · 2021Review
- Nanomaterial-mediated autophagy: coexisting hazard and health benefits in biomedicine.Particle and fibre toxicology · 2020Review
- Hydroxyl functionalized multi-walled carbon nanotubes modulate immune responses without increasing 2009 pandemic influenza A/H1N1 virus titers in infected mice.Toxicology and applied pharmacology · 2020Article
- DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles.International journal of molecular sciences · 2020Article
- No excessive mutations in transcription activator-like effector nuclease-mediated α-1,3-galactosyltransferase knockout Yucatan miniature pigs.Asian-Australasian journal of animal sciences · 2020Article
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundSubtle DNA methylation alterations mediated by carbon nanotubes (CNTs) exposure might contribute to pathogenesis and disease susceptibility. It is known that both multi-walled carbon nanotubes (MWCNTs) and single-walled carbon nanotubes (SWCNTs) interact with nucleus. Such, nuclear-CNT interaction may affect the DNA methylation effects. In order to understand the epigenetic toxicity, in particular DNA methylation alterations, of SWCNTs and short MWCNTs, we performed global/genome-wide, gene-specific DNA methylation and RNA-expression analyses after exposing human bronchial epithelial cells (16HBE14o- cell line). In addition, the presence of CNTs on/in the cell nucleus was evaluated in a label-free way using femtosecond pulsed laser microscopy.
resultsGenerally, a higher number of SWCNTs, compared to MWCNTs, was deposited at both the cellular and nuclear level after exposure. Nonetheless, both CNT types were in physical contact with the nuclei. While particle type dependency was noticed for the identified genome-wide and gene-specific alterations, no global DNA methylation alteration on 5-methylcytosine (5-mC) sites was observed for both CNTs. After exposure to MWCNTs, 2398 genes were hypomethylated (at gene promoters), and after exposure to SWCNTs, 589 CpG sites (located on 501 genes) were either hypo- (N = 493 CpG sites) or hypermethylated (N = 96 CpG sites). Cells exposed to MWCNTs exhibited a better correlation between gene promoter methylation and gene expression alterations. Differentially methylated and expressed genes induced changes (MWCNTs > SWCNTs) at different cellular pathways, such as p53 signalling, DNA damage repair and cell cycle. On the other hand, SWCNT exposure showed hypermethylation on functionally important genes, such as SKI proto-oncogene (SKI), glutathione S-transferase pi 1 (GTSP1) and shroom family member 2 (SHROOM2) and neurofibromatosis type I (NF1), which the latter is both hypermethylated and downregulated.
conclusionAfter exposure to both types of CNTs, epigenetic alterations may contribute to toxic or repair response. Moreover, our results suggest that the observed differences in the epigenetic response depend on particle type and differential CNT-nucleus interactions.
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