ArticleBMC plant biology2018
Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
- Meta-analysis of the effects of overexpression of WRKY transcription factors on plant responses to drought stress.BMC genetics · 2019Pooled it
- Environmental Factors, Developmental Genes and Oxidative Stress Determine Inter-Species Variability in Seed Longevity in Salicaceae.Plants (Basel, Switzerland) · 2025Article
- Unravelling the role of WRKY transcription factors in leaf senescence: Genetic and molecular insights.Journal of advanced research · 2025Review
- Somatic Embryogenesis and Genetic Transformation ofPlants (Basel, Switzerland) · 2025Article
- Genome-wide identification and transcriptional expression profiles of the transcription factor WRKY in Gentiana macrophylla.Molecular biology reports · 2025Article
- Genome-Wide Identification and Expression Profiling of Dehydration-Responsive Element-Binding Family Genes in Flax (International journal of molecular sciences · 2025Article
- Chromosome-scale genome assembly of Apocynum pictum, a drought-tolerant medicinal plant from the Tarim Basin.G3 (Bethesda, Md.) · 2024Article
- Overexpression of Wheat Selenium-Binding Protein GeneInternational journal of molecular sciences · 2024Article
- The Potential Role of Genic-SSRs in Driving Ecological Adaptation Diversity inInternational journal of molecular sciences · 2024Article
- Identification of WRKY gene family inPeerJ · 2024Article
- Physiological and molecular characteristics associated with the anti-senescence inPhotosynthetica · 2024Article
- Integrating Full-Length Transcriptome and RNA Sequencing of Siberian Wildrye (Plants (Basel, Switzerland) · 2023Article
- Genome-Wide Analysis of WRKY Transcription Factors Involved in Abiotic Stress and ABA Response inInternational journal of molecular sciences · 2023Article
- Isolation and Functional Analysis ofInternational journal of molecular sciences · 2022Article
- Genome-wide analysis of the DREB family genes and functional identification of the involvement of BrDREB2B in abiotic stress in wucai (Brassica campestris L.).BMC genomics · 2022Article
- SbWRKY55 regulates sorghum response to saline environment by its dual role in abscisic acid signaling.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Genome-Wide Analysis of the Banana WRKY Transcription Factor Gene Family Closely Related to Fruit Ripening and Stress.Plants (Basel, Switzerland) · 2022Article
- Genome-wide identification of the Liriodendron chinense WRKY gene family and its diverse roles in response to multiple abiotic stress.BMC plant biology · 2022Article
- Selection and validation of reference genes for RT-qPCR analysis of different organs at various development stages inOpen life sciences · 2022Article
- Cloning and prokaryotic expression ofOpen life sciences · 2022Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundWRKY transcription factors, one of the largest families of transcriptional regulators in plants, play important roles in plant development and various stress responses. The WRKYs of Caragana intermedia are still not well characterized, although many WRKYs have been identified in various plant species.
resultsWe identified 53 CiWRKY genes from C. intermedia transcriptome data, 28 of which exhibited complete open reading frames (ORFs). These CiWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and zinc finger motifs. Conserved domain analysis showed that the CiWRKY proteins contain a highly conserved WRKYGQK motif and two variant motifs (WRKYGKK and WKKYEEK). The subcellular localization of CiWRKY26 and CiWRKY28-1 indicated that these two proteins localized exclusively to nuclei, supporting their role as transcription factors. The expression patterns of the 28 CiWRKYs with complete ORFs were examined through quantitative real-time PCR (qRT-PCR) in various tissues and under different abiotic stresses (drought, cold, salt, high-pH and abscisic acid (ABA)). The results showed that each CiWRKY responded to at least one stress treatment. Furthermore, overexpression of CiWRKY75-1 and CiWRKY40-4 in Arabidopsis thaliana suppressed the drought stress tolerance of the plants and delayed leaf senescence, respectively.
conclusionsFifty-three CiWRKY genes from the C. intermedia transcriptome were identified and divided into three groups via phylogenetic analysis. The expression patterns of the 28 CiWRKYs under different abiotic stresses suggested that each CiWRKY responded to at least one stress treatment. Overexpression of CiWRKY75-1 and CiWRKY40-4 suppressed the drought stress tolerance of Arabidopsis and delayed leaf senescence, respectively. These results provide a basis for the molecular mechanism through which CiWRKYs mediate stress tolerance.
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