Evidence map›Paper›PMID 29425767›Full record

ArticleVirology2018

Proteomic profiling of HIV-infected T-cells by SWATH mass spectrometry.

Jason DeBoer, Melinda S Wojtkiewicz, Nicole Haverland, Yan Li, Emma Harwood, Emily Leshen, Joseph W George, Pawel Ciborowski, Michael Belshan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. CD4Physiological reports · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Jason DeBoerDepartment of Medical Microbiology and Immunology, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA.
Melinda S WojtkiewiczDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.
Nicole HaverlandDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.
Yan LiDepartment of Medical Microbiology and Immunology, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA.
Emma HarwoodDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.
Emily LeshenDepartment of Medical Microbiology and Immunology, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA.
Joseph W GeorgeDepartment of Medical Microbiology and Immunology, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA.
Pawel CiborowskiDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA; The Nebraska Center for Virology, University of Nebraska, Lincoln, NE, USA.
Michael BelshanDepartment of Medical Microbiology and Immunology, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA; The Nebraska Center for Virology, University of Nebraska, Lincoln, NE, USA. Electronic address: michaelbelshan@creighton.edu.
Creighton University · USUniversity of Nebraska Medical Center · USUniversity of Nebraska–Lincoln · US

Funding

Therapeutics Core (Page 286)P30MH062261 · NIMH · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI BUCH, SHILPA J., FOX, HOWARD S · 2000 to 2021
$37.7M
Transcriptional Regulation, the Nuclear Proteome, and HIV/Meth/cART: From ProfiliR01DA030962 · NIDA · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI CIBOROWSKI, PAWEL S, FOX, HOWARD S · 2010 to 2014
$3.1M
Macrophage, Meth, HIV and Histones: An InterplayR01DA043258 · NIDA · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI CIBOROWSKI, PAWEL S, FOX, HOWARD S · 2016 to 2020
$3.1M
A Proteomic and Biochemical Study of HIV-1 Nucleoprotein ComplexesR01AI080348 · NIAID · CREIGHTON UNIVERSITY · PI BELSHAN, MICHAEL A · 2010 to 2014
$1.8M
NIAID NIH HHS R01 AI080348NIDA NIH HHS R01 DA030962NIDA NIH HHS R01 DA043258NIMH NIH HHS P30 MH062261
6 · The paper itself

Abstract

Viral pathogenesis results from changes in host cells due to virus usurpation of the host cell and the innate cellular responses to thwart infection. We measured global changes in protein expression and localization in HIV-1 infected T-cells using subcellular fractionation and the Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS) proteomic platform. Eight biological replicates were performed in two independent experimental series. In silico merging of both experiments identified 287 proteins with altered expression (p < .05) between control and infected cells- 172 in the cytoplasm, 84 in the membrane, and 31 in nuclei. 170 of the proteins are components of the NIH HIV interaction database. Multiple Reaction Monitoring and traditional immunoblotting validated the altered expression of several factors during infection. Numerous factors were found to affect HIV infection in gain- and loss-of-expression infection assays, including the intermediate filament vimentin which was found to be required for efficient infection.

Indexed as

HIV-1HIV InfectionsHumansProteinsProteomicsTandem Mass SpectrometryT-LymphocytesProteinsHIV-1Host response to infectionProteomicsSWATH-MSVimentin

Identifiers

PMID29425767
PMCPMC5836514
OpenAlexW2792945463

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.