Evidence map›Paper›PMID 29425673›Full record

ArticleBiochimica et biophysica acta. Biomembranes2018

Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1.

Zhengrong Yang, Ellen Hildebrandt, Fan Jiang, Andrei A Aleksandrov, Netaly Khazanov, Qingxian Zhou, Jianli An, Andrew T Mezzell, Bala M Xavier, Haitao Ding and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta. Biomembranes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Proteostasis landscapes of cystic fibrosis variants reveal drug response vulnerability.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
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  9. Article
  10. Whole-Exome Sequencing IdentifiedFrontiers in genetics · 2021
    Article
  11. Towards next generation therapies for cystic fibrosis: Folding, function and pharmacology of CFTR.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2020
    Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Zhengrong YangDepartment of Chemistry, University of Alabama at Birmingham, Birmingham, AL, USA.
Ellen HildebrandtDepartment of Cell Biology and Biochemistry, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, 3601 4th Street, Stop 6540, Lubbock, TX 79430, USA.
Fan JiangDepartment of Medicine, University of Alabama at Birmingham, 701 19th Street South, Birmingham, AL 35294-0007, USA.
Andrei A AleksandrovDepartment of Biochemistry and Biophysics and Cystic Fibrosis Treatment and Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Netaly KhazanovDepartment of Chemistry, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
Qingxian ZhouDepartment of Chemistry, University of Alabama at Birmingham, Birmingham, AL, USA.
Jianli AnDepartment of Chemistry, University of Alabama at Birmingham, Birmingham, AL, USA.
Andrew T MezzellDepartment of Medicine, University of Alabama at Birmingham, 701 19th Street South, Birmingham, AL 35294-0007, USA.
Bala M XavierDepartment of Cell Biology and Biochemistry, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, 3601 4th Street, Stop 6540, Lubbock, TX 79430, USA.
Haitao DingDepartment of Medicine, University of Alabama at Birmingham, 701 19th Street South, Birmingham, AL 35294-0007, USA.
John R RiordanDepartment of Biochemistry and Biophysics and Cystic Fibrosis Treatment and Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Hanoch SenderowitzDepartment of Chemistry, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
John C KappesDepartment of Medicine, University of Alabama at Birmingham, 701 19th Street South, Birmingham, AL 35294-0007, USA; Birmingham Veterans Affairs Medical Center, Research Service, Birmingham, AL 35233, USA.
Christie G BrouilletteDepartment of Chemistry, University of Alabama at Birmingham, Birmingham, AL, USA. Electronic address: christie@uab.edu.
Ina L UrbatschDepartment of Cell Biology and Biochemistry, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, 3601 4th Street, Stop 6540, Lubbock, TX 79430, USA. Electronic address: ina.urbatsch@ttuhsc.edu.

Funding

ROS Modulation of Innate and Adaptive Immunity in RAP30AR048311 · NIAMS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI MOUNTZ, JOHN D · 2001 to 2017
$9.5M
MicroCal Auto-iTC200; automated high sensitivity isothermal titration calorimetryS10RR026478 · NCRR · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI BROUILLETTE, CHRISTIE G. · 2010 to 2010
$250k
NCRR NIH HHS S10 RR026478NIAMS NIH HHS P30 AR048311NIH HHS 1S10RR026478NIH HHS P30 AI027667
6 · The paper itself

Abstract

The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is an ABC transporter containing two transmembrane domains forming a chloride ion channel, and two nucleotide binding domains (NBD1 and NBD2). CFTR has presented a formidable challenge to obtain monodisperse, biophysically stable protein. Here we report a comprehensive study comparing effects of single and multiple NBD1 mutations on stability of both the NBD1 domain alone and on purified full length human CFTR. Single mutations S492P, A534P, I539T acted additively, and when combined with M470V, S495P, and R555K cumulatively yielded an NBD1 with highly improved structural stability. Strategic combinations of these mutations strongly stabilized the domain to attain a calorimetric T

Indexed as

Cystic Fibrosis Transmembrane Conductance RegulatorMutationProtein Interaction Domains and MotifsAdenosine TriphosphatasesAnimalsBinding SitesCHO CellsCricetinaeCricetulusEnzyme StabilityHEK293 CellsHumansModels, MolecularMutagenesis, Site-DirectedNucleotidesProtein BindingAdenosine TriphosphatasesCFTR protein, humanCystic Fibrosis Transmembrane Conductance RegulatorNucleotidesABC transportersATP hydrolysisCFTRNBD1Protein unfoldingStabilizing mutationsThermal stability

Identifiers

PMID29425673
PMCPMC6319260

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.