ArticlePLoS neglected tropical diseases2018
Unique pharmacological properties of serotoninergic G-protein coupled receptors from cestodes.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Serotonin signaling modulates growth and motility in juvenile Fasciola hepatica.PLoS neglected tropical diseases · 2026Article
- Extracts and Terpenoids fromMolecules (Basel, Switzerland) · 2024Article
- Article
- Molecular characterization of the serotonergic transporter from the cestode Echinococcus granulosus: pharmacology and potential role in the nervous system.Parasitology research · 2022Article
- A validated high-throughput method for assaying rat lungworm (Parasitology · 2022Article
- The interplay of helminthic neuropeptides and proteases in parasite survival and host immunomodulation.Biochemical Society transactions · 2022Review
- Article
- The potential for histone deacetylase (HDAC) inhibitors as cestocidal drugs.PLoS neglected tropical diseases · 2021Article
- Serotonin stimulates Echinococcus multilocularis larval development.Parasites & vectors · 2021Article
- Characterization of a new type of neuronal 5-HT G- protein coupled receptor in the cestode nervous system.PloS one · 2021Article
- Cestode strobilation: prediction of developmental genes and pathways.BMC genomics · 2020Article
- Transcriptomic Features of Echinococcus granulosus Protoscolex during the Encystation Process.The Korean journal of parasitology · 2020Article
- Histone deacetylase enzymes as potential drug targets of Neglected Tropical Diseases caused by cestodes.International journal for parasitology. Drugs and drug resistance · 2019Article
- Comparison of different staining methods for determination of viability on Mesocestoides vogae tetrathyridia.Parasitology research · 2019Article
- Structure-activity profiling of alkaloid natural product pharmacophores against a Schistosoma serotonin receptor.International journal for parasitology. Drugs and drug resistance · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 5 institutions in 4 countries.
Funding
Abstract
backgroundCestodes are a diverse group of parasites, some of them being agents of neglected diseases. In cestodes, little is known about the functional properties of G protein coupled receptors (GPCRs) which have proved to be highly druggable targets in other organisms. Notably, serotoninergic G-protein coupled receptors (5-HT GPCRs) play major roles in key functions like movement, development and reproduction in parasites. METHODOLOGY/PRINCIPAL
findingsThree 5-HT GPCRs from Echinococcus granulosus and Mesocestoides corti were cloned, sequenced, bioinformatically analyzed and functionally characterized. Multiple sequence alignment with other GPCRs showed the presence of seven transmembrane segments and conserved motifs but interesting differences were also observed. Phylogenetic analysis grouped these new sequences within the 5-HT7 clade of GPCRs. Molecular modeling showed a striking resemblance in the spatial localization of key residues with their mammalian counterparts. Expression analysis using available RNAseq data showed that both E. granulosus sequences are expressed in larval and adult stages. Localization studies performed in E. granulosus larvae with a fluorescent probe produced a punctiform pattern concentrated in suckers. E. granulosus and M. corti larvae showed an increase in motility in response to serotonin. Heterologous expression revealed elevated levels of cAMP production in response to 5-HT and two of the GPCRs showed extremely high sensitivity to 5-HT (picomolar range). While each of these GPCRs was activated by 5-HT, they exhibit distinct pharmacological properties (5-HT sensitivity, differential responsiveness to ligands). CONCLUSIONS/SIGNIFICANCE: These data provide the first functional report of GPCRs in parasitic cestodes. The serotoninergic GPCRs characterized here may represent novel druggable targets for antiparasitic intervention.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.