ArticlePloS one2018
Identification and characterization of influenza A viruses in selected domestic animals in Kenya, 2010-2012.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.
- Influenza A and D Viruses in Non-Human Mammalian Hosts in Africa: A Systematic Review and Meta-Analysis.Viruses · 2021Pooled it
- Review
- Human Seasonal Influenza Viruses in Swine Workers in Lagos, Nigeria: Consequences for Animal and Public Health.Viruses · 2023Article
- A systematic review of influenza A virus prevalence and transmission dynamics in backyard swine populations globally.Porcine health management · 2022Review
- A Systematic Review Analyzing the Prevalence and Circulation of Influenza Viruses in Swine Population Worldwide.Pathogens (Basel, Switzerland) · 2020Review
- Systematic Review of Important Viral Diseases in Africa in Light of the 'One Health' Concept.Pathogens (Basel, Switzerland) · 2020Article
- Status and gaps of research on respiratory disease pathogens of swine in Africa.Porcine health management · 2020Review
- Detection of pandemic influenza A/H1N1/pdm09 virus among pigs but not in humans in slaughterhouses in Kenya, 2013-2014.BMC research notes · 2019Article
- Seroprevalence of influenza A virus in pigs and low risk of acute respiratory illness among pig workers in Kenya.Environmental health and preventive medicine · 2019Article
- Molecular and serological prevalence of influenza A viruses in poultry and poultry farmers in the Ashanti region, Ghana.Infection ecology & epidemiology · 2019Article
- Complete genome sequencing of H1N1pdm09 swine influenza isolates from Nigeria reveals likely reverse zoonotic transmission at the human-animal interface in intensive piggery.Infection ecology & epidemiology · 2019Article
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11 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundInfluenza A virus subtypes in non-human hosts have not been characterized in Kenya. We carried out influenza surveillance in selected domestic animals and compared the virus isolates with isolates obtained in humans during the same period.
methodsWe collected nasal swabs from pigs, dogs and cats; oropharyngeal and cloacal swabs from poultry; and blood samples from all animals between 2010 and 2012. A standardized questionnaire was administered to farmers and traders. Swabs were tested for influenza A by rtRT-PCR, virus isolation and subtyping was done on all positive swabs. All sera were screened for influenza A antibodies by ELISA, and positives were evaluated by hemagglutination inhibition (HI). Full genome sequencing was done on four selected pig virus isolates.
resultsAmong 3,798 sera tested by ELISA, influenza A seroprevalence was highest in pigs (15.9%; 172/1084), 1.2% (3/258) in ducks, 1.4% (1/72) in cats 0.6% (3/467) in dogs, 0.1% (2/1894) in chicken and 0% in geese and turkeys. HI testing of ELISA-positive pig sera showed that 71.5% had positive titers to A/California/04/2009(H1N1). Among 6,289 swabs tested by rRT-PCR, influenza A prevalence was highest in ducks [1.2%; 5/423] and 0% in cats and turkeys. Eight virus isolates were obtained from pig nasal swabs collected in 2011 and were determined to be A(H1N1)pdm09 on subtyping. On phylogenetic analysis, four hemagglutinin segments from pig isolates clustered together and were closely associated with human influenza viruses that circulated in Kenya in 2011.
conclusionInfluenza A(H1N1)pdm09 isolated in pigs was genetically similar to contemporary human pandemic influenza virus isolates. This suggest that the virus was likely transmitted from humans to pigs, became established and circulated in Kenyan pig populations during the study period. Minimal influenza A prevalence was observed in the other animals studied.
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