Evidence map›Paper›PMID 29424951›Full record

ArticleChembiochem : a European journal of chemical biology2018

Synthesis and Biological Evaluation of Fluorescent Bryostatin Analogues.

Thomas J Cummins, Noemi Kedei, Agnes Czikora, Nancy E Lewin, Sharon Kirk, Mark E Petersen, Kevin M McGowan, Jin-Qiu Chen, Xiaoling Luo, Randall C Johnson and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 1 country.

Thomas J CumminsUniversity of Utah, Department of Chemistry, 315 South 1400 East, Room 2020, Salt Lake City, UT, 84112, USA.
Noemi KedeiLaboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive, Room 4048, Bethesda, MD, 20892, USA.
Agnes CzikoraLaboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive, Room 4048, Bethesda, MD, 20892, USA.
Nancy E LewinLaboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive, Room 4048, Bethesda, MD, 20892, USA.
Sharon KirkUniversity of Utah, Department of Chemistry, 315 South 1400 East, Room 2020, Salt Lake City, UT, 84112, USA.
Mark E PetersenUniversity of Utah, Department of Chemistry, 315 South 1400 East, Room 2020, Salt Lake City, UT, 84112, USA.
Kevin M McGowanUniversity of Utah, Department of Chemistry, 315 South 1400 East, Room 2020, Salt Lake City, UT, 84112, USA.
Jin-Qiu ChenCollaborative Protein Technology Resource, Center for Cancer Research, National Cancer Institute, 37 Convent Drive, Room 1044, Bethesda, MD, 20892, USA.
Xiaoling LuoCollaborative Protein Technology Resource, Center for Cancer Research, National Cancer Institute, 37 Convent Drive, Room 1044, Bethesda, MD, 20892, USA.
Randall C JohnsonAdvanced Biomedical and Computational Sciences Biomedical Informatics, and Data Science (BIDS), Directorate Frederick National Laboratory for Cancer Research (FNLCR), Leidos Biomedical Research, Inc., Building 430, Miller Drive, Fort Detrick, Frederick, MD, 21702, USA.
Sarangan RavichandranAdvanced Biomedical and Computational Sciences Biomedical Informatics, and Data Science (BIDS), Directorate Frederick National Laboratory for Cancer Research (FNLCR), Leidos Biomedical Research, Inc., Building 430, Miller Drive, Fort Detrick, Frederick, MD, 21702, USA.
Peter M BlumbergLaboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive, Room 4048, Bethesda, MD, 20892, USA.
Gary E KeckUniversity of Utah, Department of Chemistry, 315 South 1400 East, Room 2020, Salt Lake City, UT, 84112, USA.
Center for Cancer Research · USUniversity of Utah · USFrederick National Laboratory for Cancer Research · USLeidos (United States) · USNational Cancer Institute · US

Funding

Molecular Mechanism of Action of Phorbol EsterZIABC005270 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI BLUMBERG, PETER · 2009 to 2018
$18.4M
Spatial Imaging Technology ResourceZICBC011434 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI MALIK, MARIAM · 2012 to 2025
$12.8M
CHIRAL APPROACHES TO NATURAL PRODUCT SYNTHESISR01GM028961 · NIGMS · UNIVERSITY OF UTAH · PI KECK, GARY E · 1985 to 2014
$5.5M
MOLECULAR MECHANISM OF ACTION OF PHORBOL ESTER TUMOR PROMOTERSZ01BC005270 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI BLUMBERG, PETER · 1996 to 2008
$4.3M
Intramural NIH HHS Z01 BC005270NIGMS NIH HHS R01 GM028961
6 · The paper itself

Abstract

To investigate the cellular distribution of tumor-promoting vs. non-tumor-promoting bryostatin analogues, we synthesized fluorescently labeled variants of two bryostatin derivatives that have previously shown either phorbol ester-like or bryostatin-like biological activity in U937 leukemia cells. These new fluorescent analogues both displayed high affinity for protein kinase C (PKC) binding and retained the basic properties of the parent unlabeled compounds in U937 assays. The fluorescent compounds showed similar patterns of intracellular distribution in cells, however; this argues against an existing hypothesis that various patterns of intracellular distribution are responsible for differences in biological activity. Upon further characterization, the fluorescent compounds revealed a slow rate of cellular uptake; correspondingly, they showed reduced activity for cellular responses that were only transient upon treatment with phorbol ester or bryostatin 1.

Indexed as

BryostatinsFluorescent DyesHumansPhorbol EstersProtein BindingProtein Kinase CU937 CellsBryostatinsFluorescent DyesPhorbol EstersProtein Kinase Cbryostatinfluorescent probesMerleprotein kinase Cpyran annulation

Identifiers

PMID29424951
PMCPMC6980389
OpenAlexW2793800983

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.