ArticleMolecular microbiology2018
Interference of transcription across H-NS binding sites and repression by H-NS.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- How mutagenesis and laboratory conditions affect the genome of the metal-resistant bacteriumJournal of bacteriology · 2026Article
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- Spatio-temporal organization of theEcoSal Plus · 2024Review
- Bacterial chromatin proteins, transcription, and DNA topology: Inseparable partners in the control of gene expression.Molecular microbiology · 2024Review
- Genetic Approaches to Study the Interplay Between Transcription and Nucleoid-Associated Proteins in Escherichia coli.Methods in molecular biology (Clifton, N.J.) · 2024Article
- In Vitro Transcription Assay to Quantify Effects of H-NS Filaments on RNA Chain Elongation by RNA Polymerase.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The environmentally-regulated interplay between local three-dimensional chromatin organisation and transcription of proVWX in E. coli.Nature communications · 2023Article
- Pervasive transcription enhances the accessibility of H-NS-silenced promoters and generates bistability inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- High Abundance of Transcription Regulators Compacts the Nucleoid in Escherichia coli.Journal of bacteriology · 2022Article
- DissectingJournal of bacteriology · 2021Article
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- Divergently Transcribed ncRNAs inFrontiers in molecular biosciences · 2021Article
- The transcription regulator and c-di-GMP phosphodiesterase PdeL represses motility inJournal of bacteriology · 2020Article
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- NusG prevents transcriptional invasion of H-NS-silenced genes.PLoS genetics · 2019Article
- Transcription of Bacterial Chromatin.Journal of molecular biology · 2019Review
- Organization of DNA in Mammalian Mitochondria.International journal of molecular sciences · 2019Review
- High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription.Cell systems · 2019Article
- StpA and Hha stimulate pausing by RNA polymerase by promoting DNA-DNA bridging of H-NS filaments.Nucleic acids research · 2018Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Nucleoid-associated protein H-NS represses transcription by forming extended DNA-H-NS complexes. Repression by H-NS operates mostly at the level of transcription initiation. Less is known about how DNA-H-NS complexes interfere with transcription elongation. In vitro H-NS has been shown to enhance RNA polymerase pausing and to promote Rho-dependent termination, while in vivo inhibition of Rho resulted in a decrease of the genome occupancy by H-NS. Here we show that transcription directed across H-NS binding regions relieves H-NS (and H-NS/StpA) mediated repression of promoters in these regions. Further, we observed a correlation of transcription across the H-NS-bound region and de-repression. The data suggest that the transcribing RNA polymerase is able to remodel the H-NS complex and/or dislodge H-NS from the DNA and thus relieve repression. Such an interference of transcription and H-NS mediated repression may imply that poorly transcribed AT-rich loci are prone to be repressed by H-NS, while efficiently transcribed loci escape repression.
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