Evidence map›Paper›PMID 29423859›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2018

Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains.

Sudhir Kumar Rai, Angela Atwood-Moore, Henry L Levin

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Sudhir Kumar RaiSection on Eukaryotic Transposable Elements, Division of Molecular and Cellular Biology, The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Angela Atwood-MooreSection on Eukaryotic Transposable Elements, Division of Molecular and Cellular Biology, The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Henry L LevinSection on Eukaryotic Transposable Elements, Division of Molecular and Cellular Biology, The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA. levinh@mail.nih.gov.
National Institutes of Health · USUniversity of Florida · US

Funding

Regulation And Function Of RetroelementsZIAHD001009 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI LEVIN, HENRY L. · 2009 to 2025
$21.8M
Development and application of transposable element technologyZIAHD008957 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI LEVIN, HENRY L. · 2016 to 2025
$6.0M
REGULATION AND FUNCTION OF RETROELEMENTSZ01HD001009 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI LEVIN, HENRY L. · 1993 to 2008
$2.7M
Intramural NIH HHS Z01 HD001009
6 · The paper itself

Abstract

We present an efficient and organized method of lithium acetate and polyethylene glycol-based transformation of plasmid DNA into the commercially available collection of Schizosaccharomyces pombe with single-gene deletions. We also describe how to prepare a duplicate collection of the deletion strains in order to preserve the longevity of the master set. These protocols are adapted to the 96-well format of the 3004 strains of the Version 2.0 Bioneer set but can also be used for later releases of the collection. This transformation method typically yields efficiencies in the range between 1.0 × 10

Indexed as

Gene DeletionTransformation, GeneticSchizosaccharomycesTransfectionCommercial deletion libraryFission yeastLithium acetatePolyethylene glycolSchizosaccharomyces pombeTf1Transformation

Identifiers

PMID29423859
PMCPMC6390284
OpenAlexW2790006334

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.