Evidence map›Paper›PMID 29423855›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2018

In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe.

Jinpu Yang, Fei Li

Abstract readReview
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Jinpu YangDepartment of Biology, New York University, New York, NY, USA.
Fei LiDepartment of Biology, New York University, New York, NY, USA. fl43@nyu.edu.
New York University · US

Funding

Molecular Basis of Centromere Specification and InheritanceR01GM106037 · NIGMS · NEW YORK UNIVERSITY · PI LI, FEI · 2014 to 2018
$1.5M
NIGMS NIH HHS R01 GM106037
6 · The paper itself

Abstract

Chromatin-associated proteins play critical roles in many cellular processes, including gene expression, epigenetic regulation, DNA repair, recombination, and replication. Especially, epigenetic landscape, shaped by a variety of chromatin-binding proteins, is dynamic and regulated in a context-dependent manner. In situ chromatin-binding assay is a powerful but simple tool to investigate how proteins, such as epigenetic components, associate with chromatin. This approach relies on the fact that chromatin bound proteins are more resistant to detergent extraction. Here, we describe a protocol for the in situ chromatin-binding assay used in Schizosaccaromyces pombe.

Indexed as

Biological AssayChromatinSchizosaccharomycesSchizosaccharomyces pombe ProteinsChromatinSchizosaccharomyces pombe ProteinsCENP-AEpigeneticsFission yeastGFPH3In situ chromatin bindingSchizosaccaromyces pombe

Identifiers

PMID29423855
PMCPMC6339571
OpenAlexW2792508729

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.