Evidence map›Paper›PMID 29423852›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2018

Antibody Pull-Down Experiments in Fission Yeast.

Qianhua Dong, Fei Li

Abstract readReview
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Rex1BD and the 14-3-3 protein control heterochromatin organization at tandem repeats by linking RNAi and HDAC.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Qianhua DongDepartment of Biology, New York University, New York, NY, USA.
Fei LiDepartment of Biology, New York University, New York, NY, USA. fl43@nyu.edu.

Funding

Molecular Basis of Centromere Specification and InheritanceR01GM106037 · NIGMS · NEW YORK UNIVERSITY · PI LI, FEI · 2014 to 2018
$1.5M
NIGMS NIH HHS R01 GM106037
6 · The paper itself

Abstract

Proteins act as executors for almost all kinds of cellular processes. The majority of proteins achieve their proper functions through interacting with other proteins. Knowing the binding partners of a protein is instrumental for understanding its function. The antibody pull-down method is a powerful and common approach to detect protein-protein interactions. Here, an antibody pull-down protocol is described for detecting protein-protein interactions in fission yeast Schizosaccharomyces pombe.

Indexed as

AnimalsHumansImmunoprecipitationSchizosaccharomycesSchizosaccharomyces pombe ProteinsSchizosaccharomyces pombe ProteinsAntibodyCo-immunoprecipitationFission yeastIn vivoProtein-protein interactionPull-downSchizosaccharomyces pombe

Identifiers

PMID29423852
PMCPMC6339568

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.