ArticleApplied microbiology and biotechnology2018
The use of extracellular DNA as a proxy for specific microbial activity.
Article in Applied microbiology and biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 62 citations in OpenAlex.
- Dairy manure reception pits as reservoirs of extracellular DNA-associated antibiotic resistance genes.Microbiology spectrum · 2026Article
- State-Specific Extraction of Environmental DNA: Spike-and-Recovery Controls to Validate and Optimise Extraction Protocols.Environmental microbiology · 2025Article
- Application of MALDI TOF and DART mass spectrometry as novel tools for classification of anaerobic gut fungi strains.Analytical and bioanalytical chemistry · 2025Article
- Tracking Different States of Spiked Environmental DNA Using Multiplex Digital PCR Assays.Environmental microbiology · 2025Article
- Inside the Atacama Desert: uncovering the living microbiome of an extreme environment.Applied and environmental microbiology · 2024Article
- Extracellular Self-DNA Effects on Yeast Cell Cycle and Transcriptome during Batch Growth.Biomolecules · 2024Article
- Exploring near-infrared spectroscopy and hyperspectral imaging as novel characterization methods for anaerobic gut fungi.FEMS microbes · 2024Article
- Extracellular DNA secreted in yeast cultures is metabolism-specific and inhibits cell proliferation.Microbial cell (Graz, Austria) · 2023Article
- Patterns and determinants of the global herbivorous mycobiome.Nature communications · 2023Article
- The Origin, Function, Distribution, Quantification, and Research Advances of Extracellular DNA.International journal of molecular sciences · 2022Review
- Why eDNA fractions need consideration in biomonitoring.Molecular ecology resources · 2022Review
- Review
- Simultaneous Metabarcoding and Quantification ofMicroorganisms · 2022Article
- Impact of novel microbial secondary metabolites on the pharma industry.Applied microbiology and biotechnology · 2022Review
- No time to die: Comparative study on preservation protocols for anaerobic fungi.Frontiers in microbiology · 2022Article
- Living Lithic and Sublithic Bacterial Communities in Namibian Drylands.Microorganisms · 2021Article
- The Effect of a High-Grain Diet on the Rumen Microbiome of Goats with a Special Focus on Anaerobic Fungi.Microorganisms · 2021Article
- The masking effect of extracellular DNA and robustness of intracellular DNA in anaerobic digester NGS studies: A discriminatory study of the total DNA pool.Molecular ecology · 2021Article
- The Molecular Information About Deadwood Bacteriomes Partly Depends on the Targeted Environmental DNA.Frontiers in microbiology · 2021Article
- Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments.Pathogens (Basel, Switzerland) · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ubiquity and relevance of extracellular DNA (exDNA) are well-known and increasingly gaining importance in many fields of application such as medicine and environmental microbiology. Although sources and types of exDNA are manifold, ratios of specific DNA-molecules inside and outside of living cells can give reliable information about the activity of entire systems and of specific microbial groups or species. Here, we introduce a method to discriminate between internal (iDNA), as well as bound and free exDNA, and evaluate various DNA fractions and related ratios (ex:iDNA) regarding their applicability to be used as a fast, convenient, and reliable alternative to more tedious RNA-based activity measurements. In order to deal with microbial consortia that can be regulated regarding their activity, we tested and evaluated the proposed method in comparison to sophisticated dehydrogenase- and RNA-based activity measurements with two anaerobic microbial consortia (anaerobic fungi and syntrophic archaea and a microbial rumen consortium) and three levels of resolution (overall activity, total bacteria, methanogenic archaea). Furthermore, we introduce a 28S rRNA gene-specific primer set and qPCR protocol, targeting anaerobic fungi (Neocallimastigomycota). Our findings show that the amount of actively released free exDNA (fDNA) strongly correlates with different activity measurements and is thus suggested to serve as a proxy for microbial activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.