Evidence map›Paper›PMID 29422974›Full record

ArticleOncology letters2018

Low risk HPV-6E6 induces apoptosis in bone marrow-derived dendritic cells.

Lina Sun, Lei Zhao, Dan Li, Shuai Xu, Xuexin Hou, Zhenjun Li

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncology letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Lina SunState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China.
Lei ZhaoDepartment of Molecular Physiology and Biophysics, Holden Comprehensive Cancer Center, University of Iowa Carver College of Medicine, Iowa, IA 52242, USA.
Dan LiState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China.
Shuai XuState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China.
Xuexin HouState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China.
Zhenjun LiState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China.
National Institute for Communicable Disease Control and Prevention · CNUniversity of Iowa · US

Funding

Alcohol Induced Oxidative Stress Inhibits Recovery From Acute Lung InjuryK08AA021404 · NIAAA · EMORY UNIVERSITY · PI SUEBLINVONG, VIRANUJ · 2012 to 2016
$871k
NIAAA NIH HHS K08 AA021404
6 · The paper itself

Abstract

The present study assessed the subcellular localization and apoptotic potential of low-risk human papilloma virus 6E6 (HPV-6E6), expressed in bone marrow-derived dendritic cells (DCs). DCs were obtained from C57BL/6 mice and transfected with a pGFP-6E6 plasmid. The subcellular localization of E6 was determined by tracing green fluorescent protein (GFP) using fluorescence microscopy. Apoptosis was assessed by staining nuclei with DAPI and performing an apoptosis-based flow cytometry assay. The co-localization of E6 and p53 was also investigated using confocal microscopy. In addition, the expression of apoptosis-associated proteins was analyzed using immunoblotting. The results of the present study demonstrated that low-risk HPV-6E6 is predominantly localized in the cytoplasm of DCs. Furthermore, p53 was upregulated in DCs transfected with pGFP-6E6 and co-localized with GFP-6E6 in the cytoplasm. DCs transfected with a control pGFP plasmid did not undergo apoptosis, whereas cells transfected with pGFP-6E6 did, as indicated by the presence of cell debris and condensation of the nuclei. Furthermore, the expression of apoptosis-associated proteins, including p53, BCL2 associated X apoptosis regulator (Bax), BCL2 homologous antagonist/killer (Bak) and cytochrome

Indexed as

apoptosisgreen fluorescent proteinhuman papilloma virus-6E6location

Identifiers

PMID29422974
PMCPMC5772942
OpenAlexW2771009770

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.