ArticleScientific reports2018
Evolution of enzymes with new specificity by high-throughput screening using DmpR-based genetic circuits and multiple flow cytometry rounds.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Disentangling Metabolite Detection from Pathway Stress Using Biosensors.ACS synthetic biology · 2025Article
- Switching promotor recognition of phage RNA polymerase in silico along lab-directed evolution path.Biophysical journal · 2022Article
- Whole-cell screening of oxidative enzymes using genetically encoded sensors.Chemical science · 2021Article
- Phage-Assisted Continuous Evolution and Selection of Enzymes for Chemical Synthesis.ACS central science · 2021Article
- Directed Evolution Methods for Enzyme Engineering.Molecules (Basel, Switzerland) · 2021Review
- Directed Evolution of a Surface-Displayed Artificial Allylic Deallylase Relying on a GFP Reporter Protein.ACS catalysis · 2021Article
- An integrative approach to improving the biocatalytic reactions of whole cells expressing recombinant enzymes.World journal of microbiology & biotechnology · 2021Review
- Biosensor-Based Directed Evolution of Methanol Dehydrogenase fromInternational journal of molecular sciences · 2021Article
- Genetically Encoded Biosensor-Based Screening for Directed Bacteriophage T4 Lysozyme Evolution.International journal of molecular sciences · 2020Article
- Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system.Scientific reports · 2020Article
- C1 Compound Biosensors: Design, Functional Study, and Applications.International journal of molecular sciences · 2019Article
- Genetically encoded biosensors for lignocellulose valorization.Biotechnology for biofuels · 2019Review
- A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts.Nature communications · 2018Article
- Development of a Metabolite Sensor for High-Throughput Detection of Aldehydes inFrontiers in bioengineering and biotechnology · 2018Article
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Authors and funding
10 authors.
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Abstract
Genetic circuit-based biosensors are useful in detecting target metabolites or in vivo enzymes using transcription factors (Tx) as a molecular switch to express reporter signals, such as cellular fluorescence and antibiotic resistance. Herein, a phenol-detecting Tx (DmpR) was employed as a critical tool for enzyme engineering, specifically for the rapid analysis of numerous mutants with multiple mutations at the active site of tryptophan-indole lyase (TIL, EC 4.1.99.1). Cellular fluorescence was monitored cell-by-cell using flow cytometry to detect the creation of phenolic compounds by a new tyrosine-phenol-lyase (TPL, EC 4.1.99.2). In the TIL scaffold, target amino acids near the indole ring (Asp
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