ArticleScientific reports2018
Glucose repression can be alleviated by reducing glucose phosphorylation rate in Saccharomyces cerevisiae.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 93 citations in OpenAlex.
- Strategies and applications for alleviating carbon catabolite repression.Synthetic and systems biotechnology · 2027Review
- Glucose repression ofbioRxiv : the preprint server for biology · 2026Article
- Biotechnological applications of D-xylose-fermenting autochthonous yeasts in lignocellulosic biorefineries.Biotechnology for biofuels and bioproducts · 2026Review
- Effects of Different Feeding Modes on Growth Performance, Blood Biochemistry, and Metabolism of Yushu Yaks During the Cold Season.Animals : an open access journal from MDPI · 2026Article
- Bioprocess optimization and purification of DLA produced using food and fishery waste.RSC advances · 2026Article
- Engineering the acid-tolerant yeastFrontiers in microbiology · 2026Article
- Metabolic engineering of Saccharomyces cerevisiae for efficient utilization of pectin-rich biomass.Journal of microbiology (Seoul, Korea) · 2025Review
- Optimizing the treatment and valorization of spent coffee grounds (SCGs) through the integration of sequential pretreatment steps, enzymatic hydrolysis, and selective fermentation.Food chemistry: X · 2025Article
- Transcriptome analysis and reverse engineering verification of SNZ3Biotechnology for biofuels and bioproducts · 2025Article
- Upregulation of the gluconeogenesis pathway was observed byFood science and biotechnology · 2025Article
- New insights into carbon metabolism in SFrontiers in fungal biology · 2025Article
- The forced activation of asexual conidiation inSynthetic and systems biotechnology · 2024Article
- Independent metabolism of oligosaccharides is the keystone of synchronous utilization of cellulose and hemicellulose inPNAS nexus · 2024Article
- Compositional and temporal division of labor modulates mixed sugar fermentation by an engineered yeast consortium.Nature communications · 2024Article
- Structural and biochemical insights of xylose MFS and SWEET transporters in microbial cell factories: challenges to lignocellulosic hydrolysates fermentation.Frontiers in microbiology · 2024Review
- Quantifying intracellular glucose levels when yeast is grown in glucose media.Scientific reports · 2023Article
- An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis.Nature communications · 2023Article
- Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.Bioprocess and biosystems engineering · 2023Article
- Article
- D-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microorganisms commonly exhibit preferential glucose consumption and diauxic growth when cultured in mixtures of glucose and other sugars. Although various genetic perturbations have alleviated the effects of glucose repression on consumption of specific sugars, a broadly applicable mechanism remains unknown. Here, we report that a reduction in the rate of glucose phosphorylation alleviates the effects of glucose repression in Saccharomyces cerevisiae. Through adaptive evolution under a mixture of xylose and the glucose analog 2-deoxyglucose, we isolated a mutant strain capable of simultaneously consuming glucose and xylose. Genome sequencing of the evolved mutant followed by CRISPR/Cas9-based reverse engineering revealed that mutations in the glucose phosphorylating enzymes (Hxk1, Hxk2, Glk1) were sufficient to confer simultaneous glucose and xylose utilization. We then found that varying hexokinase expression with an inducible promoter led to the simultaneous utilization of glucose and xylose. Interestingly, no mutations in sugar transporters occurred during the evolution, and no specific transporter played an indispensable role in simultaneous sugar utilization. Additionally, we demonstrated that slowing glucose consumption also enabled simultaneous utilization of glucose and galactose. These results suggest that the rate of intracellular glucose phosphorylation is a decisive factor for metabolic regulations of mixed sugars.
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