ArticleParasites & vectors2018
Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feeding.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Functional analysis of Mu-class glutathione S-transferase HdGSTm1 from Haemaphysalis doenitzi with a focus on the roles in the tick blood sucking.Experimental & applied acarology · 2025Article
- Cystatin antibodies interfere with ovary development in Haemaphysalis doenitzi (Acari: Ixodidae).PLoS neglected tropical diseases · 2025Article
- Depicting "arms race" of Rhipicephalus microplus and its host on a single frame platform.Parasitology research · 2025Article
- A multi-omics approach for understanding blood digestion dynamics in Ixodes scapularis and identification of anti-tick vaccine targets.Ticks and tick-borne diseases · 2024Article
- The diverse functions of Mu-class Glutathione S-transferase HrGSTm1 during the development of Hyalomma rufipes with a focus on the detoxification metabolism of cyhalothrin.Parasites & vectors · 2024Article
- Key molecules regulating the blood meals ofVeterinary research forum : an international quarterly journal · 2024Article
- Purification, characterization, and enzyme kinetics of a glutathione S transferase from larvae of the camel tick Hyalomma dromedarii.Journal, genetic engineering & biotechnology · 2023Article
- Tick innate immune responses to hematophagy andFrontiers in immunology · 2023Article
- Ethanolic Extracts ofGenes · 2022Article
- Low Genetic Polymorphism in the Immunogenic Sequences ofVaccines · 2022Article
- From Bench to Field: A Guide to Formulating and Evaluating Anti-Tick Vaccines Delving beyond Efficacy to Effectiveness.Vaccines · 2021Review
- Effect of polyphenols extracted fromJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Acquired tick resistance: The trail is hot.Parasite immunology · 2021Review
- Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin EParasites & vectors · 2021Article
- Virome and Blood Meal-Associated Host Responses inFrontiers in microbiology · 2021Article
- Sequence identification and expression profile of seven Dermacentor marginatus glutathione S-transferase genes.Experimental & applied acarology · 2020Article
- Expression analysis of glutathione S-transferases and ferritins during the embryogenesis of the tickHeliyon · 2020Article
- Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis.Parasites & vectors · 2018Article
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundTicks are obligate hematophagous parasites important economically and to health. Ticks consume large amounts of blood for their survival and reproduction; however, large amounts of iron in blood could lead to oxidative stress. Ticks use several molecules such as glutathione S-transferases (GSTs), ferritins, and peroxiredoxins to cope with oxidative stress. This study aimed to identify and characterize the GSTs of the hard tick Haemaphysalis longicornis in order to determine if they have a role in coping with oxidative stress.
methodsGenes encoding GSTs of H. longicornis were isolated from the midgut CDNA library. Genes have been cloned and recombinant GSTs have been expressed. The enzymatic activities, enzyme kinetic constants, and optimal pH of the recombinant GSTs toward 1-chloro-2,4-dinitrobenzene (CDNB) were determined. The gene transcription and protein expression profiles were determined in the whole ticks and internal organs, and developmental stages using real time RT-PCR and Western blotting during blood feeding. The localization of GST proteins in organs was also observed using immunofluorescent antibody test (IFAT).
resultsWe have isolated two genes encoding GSTs (HlGST and HlGST2). The enzymatic activity toward CDNB is 9.75 ± 3.04 units/mg protein for recombinant HlGST and 11.63 ± 4.08 units/mg protein for recombinant HlGST2. Kinetic analysis of recombinant HlGST showed K
conclusionsIn summary, a novel GST of H. longicornis has been identified. Characterization of the GSTs showed that GSTs have positive correlation with the degree and localization of oxidative stress during blood-feeding. This could indicate their protective role during oxidative stress.
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