ArticlePloS one2018
Characterization of indole-3-pyruvic acid pathway-mediated biosynthesis of auxin in Neurospora crassa.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- The Fungal Pathogen Fusarium graminearum Can Hydrolyse Tryptamine-Derived Hydroxy-Cinnamic Acid Amide Defence Compounds, and Generates Highly Increased Auxin Levels in Wheat via Copper Amine-Oxidases.Molecular plant pathology · 2026Article
- Insights into the Mechanism Underlying the Symbiosis between Seagrass and a Lulworthiaceae Fungus.Microbial ecology · 2026Article
- Indole-3-Acetic Acid Biosynthesis by Endophytic Fungi and Its Association with Plant-Fungus Interactions.Journal of fungi (Basel, Switzerland) · 2026Review
- The alterations of the synthetic pathway and metabolic flux of auxin indole-3-acetic acid govern thermotolerance inMicrobiology spectrum · 2026Article
- Cloning and Functional Analysis of the Aldehyde Dehydrogenase GeneJournal of fungi (Basel, Switzerland) · 2025Article
- Indole metabolism and its role in diabetic macrovascular and microvascular complications.American heart journal plus : cardiology research and practice · 2025Review
- Sulfonation of IAA in Urtica eliminates its DR5 auxin activity.Plant cell reports · 2024Article
- A user-friendly CRISPR/Cas9 system for mutagenesis of Neurospora crassa.Scientific reports · 2024Article
- Optimization of indole acetic acid produced by plant growth promoting fungus, aided by response surface methodology.Heliyon · 2024Article
- Genetic evidence for algal auxin production iniScience · 2024Article
- Intestinal tryptophan metabolism in disease prevention and swine production.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Review
- Article
- Aminotransferase SsAro8 Regulates Tryptophan Metabolism Essential for Filamentous Growth of Sugarcane Smut FungusMicrobiology spectrum · 2022Article
- Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa.Biotechnology for biofuels and bioproducts · 2022Article
- Neuroprotective Natural Products' Regulatory Effects on Depression via Gut-Brain Axis Targeting Tryptophan.Nutrients · 2022Review
- Mixed Transcriptome Analysis Revealed the Possible Interaction Mechanisms betweenInternational journal of molecular sciences · 2021Article
- Characterization of near-isogenic lines confirmed QTL and revealed candidate genes for plant height and yield-related traits in common wheat.Molecular breeding : new strategies in plant improvement · 2021Article
- The Uniqueness of Tryptophan in Biology: Properties, Metabolism, Interactions and Localization in Proteins.International journal of molecular sciences · 2020Review
- Indole-3-acetic acid synthesized through the indole-3-pyruvate pathway promotes Candida tropicalis biofilm formation.PloS one · 2020Article
- Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand.PloS one · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants, bacteria and some fungi are known to produce indole-3-acetic acid (IAA) by employing various pathways. Among these pathways, the indole-3-pyruvic acid (IPA) pathway is the best studied in green plants and plant-associated beneficial microbes. While IAA production circuitry in plants has been studied for decades, little is known regarding the IAA biosynthesis pathway in fungal species. Here, we present the first data for IAA-producing genes and the associated biosynthesis pathway in a non-pathogenic fungus, Neurospora crassa. For this purpose, we used a computational approach to determine the genes and outlined the IAA production circuitry in N. crassa. We then validated these data with experimental evidence. Here, we describe the homologous genes that are present in the IPA pathway of IAA production in N. crassa. High-performance liquid chromatography and thin-layer chromatography unambiguously identified IAA, indole-3-lactic acid (ILA) and tryptophol (TOL) from cultures supplemented with tryptophan. Deletion of the gene (cfp) that encodes the enzyme indole-3-pyruvate decarboxylase, which converts IPA to indole-3-acetaldehyde (IAAld), results in an accumulation of higher levels of ILA in the N. crassa culture medium. A double knock-out strain (Δcbs-3;Δahd-2) for the enzyme IAAld dehydrogenase, which converts IAAld to IAA, shows a many fold decrease in IAA production compared with the wild type strain. The Δcbs-3;Δahd-2 strain also displays slower conidiation and produces many fewer conidiospores than the wild type strain.
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