ArticleArteriosclerosis, thrombosis, and vascular biology2018
Hyperoxia Causes Mitochondrial Fragmentation in Pulmonary Endothelial Cells by Increasing Expression of Pro-Fission Proteins.
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Article in Arteriosclerosis, thrombosis, and vascular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 71 citations in OpenAlex.
- Computational modeling of substrate-dependent lung mitochondrial respiration and bioenergetics in rats with different susceptibility to hyperoxia-induced ARDS.American journal of physiology. Cell physiology · 2026Article
- Hyperoxia and iron homeostasis in developing human airway smooth muscle.American journal of physiology. Lung cellular and molecular physiology · 2026Article
- Sex-specific differences in lung mitochondrial function and injury in rats exposed to hyperoxia.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Article
- Peroxisome Proliferator-Activated Receptor α Deficiency Induces Vascular Pathologies through Endothelial Senescence in Diabetic Retinopathy.The American journal of pathology · 2026Article
- Cell-Free Circulating Mitochondrial DNA Levels Following High-Frequency Jet Ventilation-A Post Hoc Analysis.Journal of clinical medicine · 2025Article
- Ferroptosis and hyperoxic lung injury: insights into pathophysiology and treatment approaches.Frontiers in pharmacology · 2025Review
- Caffeine, MitoQ, and GABA Prophylaxis of Mitochondrial Dysfunction Induced in Human Pulmonary Cells by Normobaric-Hyperoxia and Hyperbaric-Hyperoxia.Oxidative medicine and cellular longevity · 2025Article
- Mitochondria as the central regulator of cell death in bronchopulmonary dysplasia.Frontiers in physiology · 2025Review
- Activated DRP1 promotes mitochondrial fission and induces glycolysis in ATII cells under hyperoxia.Respiratory research · 2024Article
- Injectable, oxygen-releasing, thermosensitive hydrogel promotes vascularized bone formation with prolonged oxygen delivery and improved osteoinductivity.Materials today. Bio · 2024Article
- Studying the Pulmonary Endothelium in Health and Disease: An Official American Thoracic Society Workshop Report.American journal of respiratory cell and molecular biology · 2024Article
- [Role of reactive oxygen species/silent information regulator 1 in hyperoxia-induced bronchial epithelial cell injury].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2024Article
- Article
- Post-translational modifications and bronchopulmonary dysplasia.Frontiers in pediatrics · 2024Review
- Interplay of hypoxia-inducible factors and oxygen therapy in cardiovascular medicine.Nature reviews. Cardiology · 2023Review
- Genetically Encoded Biosensors for the Fluorescence Detection of OSensors (Basel, Switzerland) · 2023Review
- Article
- Oxygen-generating microparticles downregulate HIF-1α expression, increase cardiac contractility, and mitigate ischemic injury.Acta biomaterialia · 2023Article
- KRIT1: A Traffic Warden at the Busy Crossroads Between Redox Signaling and the Pathogenesis of Cerebral Cavernous Malformation Disease.Antioxidants & redox signaling · 2023Review
- Oxidative Stress and Inflammation in Acute and Chronic Lung Injuries.Antioxidants (Basel, Switzerland) · 2023Review
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Authors and funding
12 authors at 3 institutions in 2 countries.
Cui MaFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
Andreas M BeyerFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
Matthew DurandFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
Anne V CloughFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
Daling ZhuFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
Laura Norwood ToroFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
Maia TerashviliFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
Johnathan D EbbenFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
R Blake HillFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
Said H AudiFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
Meetha MedhoraFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.).
Elizabeth R JacobsFrom the College of Medical Laboratory Science and Technology, Harbin Medical University, Daqing, China (C.M., D.Z., M.M., E.R.J.); Department of Medicine (C.M., A.M.B., A.C., L.N., J.E., M.M., E.R.J.), Department of Physical Medicine and Rehabilitation (M.D.), Department of Physiology (A.M.B., M.M., E.R.J.), Department of Biochemistry (B.H.), Department of Radiation Oncology (M.M.), Department of Biophysics (N.H.), and Cardiovascular Center (M.T., C.M., A.B., M.D., M.M., E.R.J.), Medical College of Wisconsin, Milwaukee; Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee (A.C., S.H.A., M.M., E.R.J.); Department of Biomedical Engineering, Marquette University, Milwaukee (S.H.A.); and Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee (A.C.). ejacobs@mcw.edu.
Milwaukee VA Medical Center · USMarquette University · USHarbin Medical University · CN
Funding
Clincal and Translational Science AwardUL1TR000055 · NCATS · MEDICAL COLLEGE OF WISCONSIN · PI SHAKER, REZA NONE · 2012 to 2015
$11.3MStructural Biology of Mitochondrial FissionR01GM067180 · NIGMS · MEDICAL COLLEGE OF WISCONSIN · PI HILL, R BLAKE · 2004 to 2022
$6.0MDevelopment of lisinopril for post-exposure mitigation of late effects from a radU01AI107305 · NIAID · MEDICAL COLLEGE OF WISCONSIN · PI MEDHORA, MEETHA M · 2013 to 2017
$3.0MMechanism of radiation induced endovascular injury and mitigation via the Notch-Dll4 pathwayU01AI133594 · NIAID · MEDICAL COLLEGE OF WISCONSIN · PI HIMBURG, HEATHER A · 2018 to 2022
$3.0MEndothelial function in human diabetes: role of mitochondrial fission proteinsR01HL128240 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI WIDLANSKY, MICHAEL E · 2016 to 2020
$2.9MPivotal Role of Mitochondrial Telomerase in Regulation of Vascular Tone and Redox HomeostasisR01HL133029 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI BEYER, ANDREAS M · 2017 to 2021
$2.2MInnovative Biomarkers to Predict Radiation Lung InjuryR01AI101898 · NIAID · MEDICAL COLLEGE OF WISCONSIN · PI MEDHORA, MEETHA M · 2012 to 2016
$1.9MSPECT Imaging and Computational Modeling for Assessment of Acute Lung InjuryR15HL129209 · NHLBI · MARQUETTE UNIVERSITY · PI SAID H AUDI · 2015 to 2026
$1.8MNovel imaging to identify lung mitochondrial injury and predict recoveryR01HL116530 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI JACOBS, ELIZABETH R · 2013 to 2016
$1.1MDifferentiation of mitochondrial vs. nuclear function of telomeraseR21OD018306 · OD · MEDICAL COLLEGE OF WISCONSIN · PI BEYER, ANDREAS M · 2014 to 2015
$411kNovel Diagnostics to Detect Lung InjuryI01BX001681 · VA · CLEMENT J. ZABLOCKI VA MEDICAL CENTER · PI JACOBS, ELIZABETH R · 2013 to 2016
–BLRD VA I01 BX001681BLRD VA I01 BX003833NCATS NIH HHS UL1 TR000055NHLBI NIH HHS R01 HL116530NHLBI NIH HHS R01 HL128240NHLBI NIH HHS R01 HL133029NHLBI NIH HHS R15 HL129209NIAID NIH HHS R01 AI101898NIAID NIH HHS U01 AI107305NIAID NIH HHS U01 AI133594NIGMS NIH HHS R01 GM067180NIH HHS R21 OD018306
Abstract
objectiveWe explored mechanisms that alter mitochondrial structure and function in pulmonary endothelial cells (PEC) function after hyperoxia. APPROACH AND
resultsMitochondrial structures of PECs exposed to hyperoxia or normoxia were visualized and mitochondrial fragmentation quantified. Expression of pro-fission or fusion proteins or autophagy-related proteins were assessed by Western blot. Mitochondrial oxidative state was determined using mito-roGFP. Tetramethylrhodamine methyl ester estimated mitochondrial polarization in treatment groups. The role of mitochondrially derived reactive oxygen species in mt-fragmentation was investigated with mito-TEMPOL and mitochondrial DNA (mtDNA) damage studied by using ENDO III (mt-tat-endonuclease III), a protein that repairs mDNA damage. Drp-1 (dynamin-related protein 1) was overexpressed or silenced to test the role of this protein in cell survival or transwell resistance. Hyperoxia increased fragmentation of PEC mitochondria in a time-dependent manner through 48 hours of exposure. Hyperoxic PECs exhibited increased phosphorylation of Drp-1 (serine 616), decreases in Mfn1 (mitofusion protein 1), but increases in OPA-1 (optic atrophy 1). Pro-autophagy proteins p62 (LC3 adapter-binding protein SQSTM1/p62), PINK-1 (PTEN-induced putative kinase 1), and LC3B (microtubule-associated protein 1A/1B-light chain 3) were increased. Returning cells to normoxia for 24 hours reversed the increased mt-fragmentation and changes in expression of pro-fission proteins. Hyperoxia-induced changes in mitochondrial structure or cell survival were mitigated by antioxidants mito-TEMPOL, Drp-1 silencing, or inhibition or protection by the mitochondrial endonuclease ENDO III. Hyperoxia induced oxidation and mitochondrial depolarization and impaired transwell resistance. Decrease in resistance was mitigated by mito-TEMPOL or ENDO III and reproduced by overexpression of Drp-1.
conclusionsBecause hyperoxia evoked mt-fragmentation, cell survival and transwell resistance are prevented by ENDO III and mito-TEMPOL and Drp-1 silencing, and these data link hyperoxia-induced mt-DNA damage, Drp-1 expression, mt-fragmentation, and PEC dysfunction.
Indexed as
AnimalsAntioxidantsDynaminsEndothelial CellsHyperoxiaMitochondriaMitochondrial DynamicsMitochondrial ProteinsOxidative StressOxygenPulmonary ArteryRatsReactive Oxygen SpeciesUp-RegulationAntioxidantsDnm1l protein, ratDynaminsMitochondrial ProteinsOxygenReactive Oxygen Speciescell survivalendothelial cellshyperoxiamitochondriareactive oxygen species
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