ArticleFood and environmental virology2018
Effect of the Shellfish Proteinase K Digestion Method on Norovirus Capsid Integrity.
Article in Food and environmental virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Using Porcine Gastric Mucin Coated Magnetic Beads to Concentrate and Purify Infectious Human Noroviruses for Infectivity Assays with Human Intestinal Enteroids and Zebrafish.Food and environmental virology · 2026Article
- PMAxx-RT-qPCR to Determine Human Norovirus Inactivation Following High-Pressure Processing of Oysters.Food and environmental virology · 2024Article
- A Comprehensive Review for the Surveillance of Human Pathogenic Microorganisms in Shellfish.Microorganisms · 2023Review
- Optimization and performance evaluation of an automated filtration method for the recovery of SARS-CoV-2 and other viruses in wastewater.The Science of the total environment · 2023Article
- Development of an Extraction Method to Detect Hepatitis A Virus, Hepatitis E Virus, and Noroviruses in Fish Products.Microorganisms · 2023Article
- Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications - A systematic review.Water research X · 2021Article
- Optimisation of a PMAxx™-RT-qPCR Assay and the Preceding Extraction Method to Selectively Detect Infectious Murine Norovirus Particles in Mussels.Food and environmental virology · 2021Article
- Detection and disinfection of COVID-19 virus in wastewater.Environmental chemistry letters · 2021Review
- Norovirus Monitoring in Oysters Using Two Different Extraction Methods.Food and environmental virology · 2019Article
- Current and Emerging Technologies for the Detection of Norovirus from Shellfish.Foods (Basel, Switzerland) · 2019Article
- Preliminary evaluation of BioFire FilmArrayEnvironmental science and pollution research international · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Norovirus outbreaks are associated with the consumption of contaminated shellfish, and so efficient methods to recover and detect infectious norovirus in shellfish are important. The Proteinase K digestion method used to recover norovirus from shellfish, as described in the ISO 15216, would be a good candidate but its impact on the virus capsid integrity and thus infectivity was never examined. The aim of this study was to assess the impact of the Proteinase K digestion method, and of the heat treatment component of the method alone, on norovirus (genogroups I and II) and MS2 bacteriophage capsid integrity. A slightly modified version of the ISO method was used. RT-qPCR was used for virus detection following digestion of accessible viral RNA using RNases. MS2 phage infectivity was measured using a plaque assay. The effect of shellfish digestive glands (DG) on recovery was evaluated. In the presence of shellfish DG, a reduction in MS2 phage infectivity of about 1 log
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.