ArticleThe ISME journal2018
Phylogenetic clustering of small low nucleic acid-content bacteria across diverse freshwater ecosystems.
Article in The ISME journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed.
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- The community, assembly process and potential functions of bacteria with different cell size in a river.Environmental microbiome · 2026Article
- Diverse Patescibacteria assemblages and prevalence of ultra-small free-living Parcubacteria along a subterranean estuary.mSystems · 2025Article
- Effects of Salinity Fluctuation on Antimicrobial Resistance and Virulence Factor Genes of Low and High Nucleic Acid-Content Bacteria in a Marine Environment.Microorganisms · 2025Article
- Two novel Patescibacteria:mBio · 2025Article
- Reverse Osmosis in an Advanced Water Treatment Train Produces a Simple, Consistent Microbial Community.ACS ES&T engineering · 2025Article
- Assessing microbial growth in drinking water using nucleic acid content and flow cytometry fingerprinting.iScience · 2024Article
- Beyond genomics in Patescibacteria: A trove of unexplored biology packed into ultrasmall bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Microplastics alter the functioning of marine microbial ecosystems.Ecology and evolution · 2024Article
- Unde venis? Bacterial resistance from environmental reservoirs to lettuce: tracking microbiome and resistome over a growth period.FEMS microbiology ecology · 2024Article
- Riverine bacterial communities are more shaped by species sorting in intensive urban and agricultural watersheds.Frontiers in microbiology · 2024Article
- Enrichment of different taxa of the enigmatic candidate phyla radiation bacteria using a novel picolitre droplet technique.ISME communications · 2024Article
- Microbial water quality investigation through flow cytometry fingerprinting: from source to tap.Sustainable microbiology · 2024Article
- Hyperactive nanobacteria with host-dependent traits pervade Omnitrophota.Nature microbiology · 2023Article
- Genome-centric metagenomics reveals the host-driven dynamics and ecological role of CPR bacteria in an activated sludge system.Microbiome · 2023Article
- Ultra-small bacteria and archaea exhibit genetic flexibility towards groundwater oxygen content, and adaptations for attached or planktonic lifestyles.ISME communications · 2023Article
- Article
- Spatiotemporal dynamics of high and low nucleic acid-content bacterial communities in Chinese coastal seawater: assembly process, co-occurrence relationship and the ecological functions.Frontiers in microbiology · 2023Article
- Article
- Revisiting Mt Fuji's groundwater origins with helium, vanadium and environmental DNA tracers.Nature water · 2023Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we used flow cytometry (FCM) and filtration paired with amplicon sequencing to determine the abundance and composition of small low nucleic acid (LNA)-content bacteria in a variety of freshwater ecosystems. We found that FCM clusters associated with LNA-content bacteria were ubiquitous across several ecosystems, varying from 50 to 90% of aquatic bacteria. Using filter-size separation, we separated small LNA-content bacteria (passing 0.4 µm filter) from large bacteria (captured on 0.4 µm filter) and characterized communities with 16S amplicon sequencing. Small and large bacteria each represented different sub-communities within the ecosystems' community. Moreover, we were able to identify individual operational taxonomical units (OTUs) that appeared exclusively with small bacteria (434 OTUs) or exclusively with large bacteria (441 OTUs). Surprisingly, these exclusive OTUs clustered at the phylum level, with many OTUs appearing exclusively with small bacteria identified as candidate phyla (i.e. lacking cultured representatives) and symbionts. We propose that LNA-content bacteria observed with FCM encompass several previously characterized categories of bacteria (ultramicrobacteria, ultra-small bacteria, candidate phyla radiation) that share many traits including small size and metabolic dependencies on other microorganisms.
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