ArticleScientific reports2018
Efficient non-cytotoxic fluorescent staining of halophiles.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Fluorescent probes as markers of cell envelope structure and function in halophilic archaea.Scientific reports · 2026Article
- Ultrasmall oral Saccharibacteria modulate gingival immunoactivation through type IV pili and TLR2-dependent endocytosis.Nature communications · 2026Article
- Microbial single-cell applications under anoxic conditions.Applied and environmental microbiology · 2024Review
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- The parasitic lifestyle of an archaeal symbiont.Nature communications · 2024Article
- Mitochondria-Targeted Curcumin: A Potent Antibacterial Agent against Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- Instantaneous Clearing of Biofilm (iCBiofilm): an optical approach to revisit bacterial and fungal biofilm imaging.Communications biology · 2023Article
- Cultureless enumeration of live bacteria in urinary tract infection by single-cell Raman spectroscopy.Frontiers in microbiology · 2023Article
- Probing archaeal cell biology: exploring the use of dyes in the imaging ofFrontiers in microbiology · 2023Article
- Chemogenetic Tags with Probe Exchange for Live-Cell Fluorescence Microscopy.ACS chemical biology · 2021Article
- Cell division in the archaeon Haloferax volcanii relies on two FtsZ proteins with distinct functions in division ring assembly and constriction.Nature microbiology · 2021Article
- Acidocalcisomes and Polyphosphate Granules Are Different Subcellular Structures in Agrobacterium tumefaciens.Applied and environmental microbiology · 2020Article
- Quorum sensing modulates the formation of virulent Legionella persisters within infected cells.Nature communications · 2019Article
- CRISPR-mediated gene silencing reveals involvement of the archaeal S-layer in cell division and virus infection.Nature communications · 2019Article
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Authors and funding
12 authors.
Funding
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Abstract
Research on halophilic microorganisms is important due to their relation to fundamental questions of survival of living organisms in a hostile environment. Here we introduce a novel method to stain halophiles with MitoTracker fluorescent dyes in their growth medium. The method is based on membrane-potential sensitive dyes, which were originally used to label mitochondria in eukaryotic cells. We demonstrate that these fluorescent dyes provide high staining efficiency and are beneficial for multi-staining purposes due to the spectral range covered (from orange to deep red). In contrast with other fluorescent dyes used so far, MitoTracker does not affect growth rate, and remains in cells after several washing steps and several generations in cell culture. The suggested dyes were tested on three archaeal (Hbt. salinarum, Haloferax sp., Halorubrum sp.) and two bacterial (Salicola sp., Halomonas sp.) strains of halophilic microorganisms. The new staining approach provides new insights into biology of Hbt. salinarum. We demonstrated the interconversion of rod-shaped cells of Hbt. salinarium to spheroplasts and submicron-sized spheres, as well as the cytoplasmic integrity of giant rod Hbt. salinarum species. By expanding the variety of tools available for halophile detection, MitoTracker dyes overcome long-standing limitations in fluorescence microscopy studies of halophiles.
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