ArticleNature communications2018
Design of ultra-swollen lipidic mesophases for the crystallization of membrane proteins with large extracellular domains.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 85 citations in OpenAlex.
- Advancing Biotherapeutic Treatment of Ulcerative Colitis Using Lipid Mesophases: Formulation Design and Preclinical Validation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Ultrafast Intermolecular Dynamics of Nanoconfined Water in Swollen Lipid Cubic Mesophases.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- TS2CG as a Membrane Builder.Journal of chemical theory and computation · 2025Article
- Premixing enables loading of long RNA in cubic phase lipid nanoparticles.Nature communications · 2025Article
- Cryo-EM structures of prokaryotic ligand-gated ion channel GLIC provide insights into gating in a lipid environment.Nature communications · 2024Article
- Crystallization of Ethylene Plant Hormone Receptor-Screening for Structure.Biomolecules · 2024Article
- Development of Redox-Active Lyotropic Lipid Cubic Phases for Biosensing Platforms.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Lipid-based liquid crystalline materials in electrochemical sensing and nanocarrier technology.Mikrochimica acta · 2023Review
- In vitro Evaluation of Paliperidone Palmitate Loaded Cubosomes Effective for Nasal-to-Brain Delivery.International journal of nanomedicine · 2023Article
- Coupling Lipid Nanoparticle Structure and Automated Single-Particle Composition Analysis to Design Phospholipase-Responsive Nanocarriers.Advanced materials (Deerfield Beach, Fla.) · 2022Article
- Observations of phase changes in monoolein during high viscous injection.Journal of synchrotron radiation · 2022Article
- Lyotropic Liquid Crystalline Nanostructures as Drug Delivery Systems and Vaccine Platforms.Pharmaceuticals (Basel, Switzerland) · 2022Review
- ATP synthase FCellular and molecular life sciences : CMLS · 2022Review
- Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment.International journal of molecular sciences · 2022Review
- A polytherapy based approach to combat antimicrobial resistance using cubosomes.Nature communications · 2022Article
- Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Probing Water State during Lipidic Mesophases Phase Transitions.Angewandte Chemie (International ed. in English) · 2021Article
- Dynamic closed states of a ligand-gated ion channel captured by cryo-EM and simulations.Life science alliance · 2021Article
- Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED.Structure (London, England : 1993) · 2020Article
- Carbon electrode engineering for high efficiency all-inorganic perovskite solar cells.RSC advances · 2020Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In meso crystallization of membrane proteins from lipidic mesophases is central to protein structural biology but limited to membrane proteins with small extracellular domains (ECDs), comparable to the water channels (3-5 nm) of the mesophase. Here we present a strategy expanding the scope of in meso crystallization to membrane proteins with very large ECDs. We combine monoacylglycerols and phospholipids to design thermodynamically stable ultra-swollen bicontinuous cubic phases of double-gyroid (Ia3d), double-diamond (Pn3m), and double-primitive (Im3m) space groups, with water channels five times larger than traditional lipidic mesophases, and showing re-entrant behavior upon increasing hydration, of sequences Ia3d→Pn3m→Ia3d and Pn3m→Im3m→Pn3m, unknown in lipid self-assembly. We use these mesophases to crystallize membrane proteins with ECDs inaccessible to conventional in meso crystallization, demonstrating the methodology on the Gloeobacter ligand-gated ion channel (GLIC) protein, and show substantial modulation of packing, molecular contacts and activation state of the ensued proteins crystals, illuminating a general strategy in protein structural biology.
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