ArticleNature communications2018
High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 151 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
151 citing papers in PubMed.
- Comprehensive genomic analysis and database construction for Apiales.Journal of advanced research · 2026Article
- De novo chromosome-level genome assembly and annotation of the Muscovy duck (Cairina moschata).Poultry science · 2026Article
- Chromosome-level genome assembly of Siberian kale (Brassica napus subsp. pabularia).Scientific data · 2026Article
- Cookbook for plant genome sequences.BMC genomics · 2026Review
- Mutations of short tandem repeats explain abundant trait heritability in Arabidopsis.Genome biology · 2025Article
- Cross-Activity Analysis of CRISPR/Cas9 Editing in Gene Families ofCurrent issues in molecular biology · 2025Article
- Mapping-based genome size estimation.BMC genomics · 2025Article
- NAVIP: Unraveling the influence of neighboring small sequence variants on functional impact prediction.PLoS computational biology · 2025Article
- Can a plant biologist fix a thermostat?The New phytologist · 2025Review
- A full genome assembly reveals drought stress effects on gene expression and metabolite profiles in blackcurrant (Horticulture research · 2025Article
- Alternative silencing states of transposable elements in Arabidopsis associated with H3K27me3.Genome biology · 2025Article
- Exploring vaginal microbiome: from traditional methods to metagenomic next-generation sequencing-a systematic review.Frontiers in microbiology · 2025Review
- Evolution of the Correlated Genomic Variation Landscape Across a Divergence Continuum in the Genus Castanopsis.Molecular biology and evolution · 2024Article
- An overview of recent technological developments in bovine genomics.Veterinary and animal science · 2024Review
- Chromosome-level genome assembly of Aquilaria yunnanensis.Scientific data · 2024Article
- High-quality genomes of Bombax ceiba and Ceiba pentandra provide insights into the evolution of Malvaceae species and differences in their natural fiber development.Plant communications · 2024Article
- A pan-genome of 69 Arabidopsis thaliana accessions reveals a conserved genome structure throughout the global species range.Nature genetics · 2024Article
- A chromosome-scale assembly reveals chromosomal aberrations and exchanges generating genetic diversity in Coffea arabica germplasm.Nature communications · 2024Article
- Full-length transcriptome sequencing provides insights into alternative splicing under cold stress in peanut.Frontiers in plant science · 2024Article
- Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein.Parasites & vectors · 2023Article
91 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
The handheld Oxford Nanopore MinION sequencer generates ultra-long reads with minimal cost and time requirements, which makes sequencing genomes at the bench feasible. Here, we sequence the gold standard Arabidopsis thaliana genome (KBS-Mac-74 accession) on the bench with the MinION sequencer, and assemble the genome using typical consumer computing hardware (4 Cores, 16 Gb RAM) into chromosome arms (62 contigs with an N50 length of 12.3 Mb). We validate the contiguity and quality of the assembly with two independent single-molecule technologies, Bionano optical genome maps and Pacific Biosciences Sequel sequencing. The new A. thaliana KBS-Mac-74 genome enables resolution of a quantitative trait locus that had previously been recalcitrant to a Sanger-based BAC sequencing approach. In summary, we demonstrate that even when the purpose is to understand complex structural variation at a single region of the genome, complete genome assembly is becoming the simplest way to achieve this goal.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.