Evidence map›Paper›PMID 29415652›Full record

ArticleBMC genomics2018

Transcriptomic changes in Cucurbita pepo fruit after cold storage: differential response between two cultivars contrasting in chilling sensitivity.

F Carvajal, R Rosales, F Palma, S Manzano, J Cañizares, M Jamilena, D Garrido

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
6.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 29 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

F CarvajalDepartment of Plant Physiology, Facultad de Ciencias, University of Granada, Fuentenueva s/n, 18071, Granada, Spain.
R RosalesDepartment of Plant Physiology, Facultad de Ciencias, University of Granada, Fuentenueva s/n, 18071, Granada, Spain.
F PalmaDepartment of Plant Physiology, Facultad de Ciencias, University of Granada, Fuentenueva s/n, 18071, Granada, Spain.
S ManzanoDepartment of Biology and Geology, Agrifood Campus of International Excellence (CeiA3), CIAIMBITAL, University of Almería, La Cañada de San Urbano s/n, 04120, Almería, Spain.
J CañizaresInstitute for the Conservation and Breeding of Agricultural Biodiversity (COMAV-UPV), Universitat Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain.
M JamilenaDepartment of Biology and Geology, Agrifood Campus of International Excellence (CeiA3), CIAIMBITAL, University of Almería, La Cañada de San Urbano s/n, 04120, Almería, Spain.
D GarridoDepartment of Plant Physiology, Facultad de Ciencias, University of Granada, Fuentenueva s/n, 18071, Granada, Spain. dgarrido@ugr.es.
Universidad de Granada · ESUniversity of Almería · ESUniversitat Politècnica de València · ES

Funding

Ministerio de Economía y Competitividad AGL2014-54598-C2
6 · The paper itself

Abstract

backgroundZucchini fruit is susceptible to chilling injury (CI), but the response to low storage temperature is cultivar dependent. Previous reports about the response of zucchini fruit to chilling storage have been focused on the physiology and biochemistry of this process, with little information about the molecular mechanisms underlying it. In this work, we present a comprehensive analysis of transcriptomic changes that take place after cold storage in zucchini fruit of two commercial cultivars with contrasting response to chilling stress.

resultsRNA-Seq analysis was conducted in exocarp of fruit at harvest and after 14 days of storage at 4 and 20 °C. Differential expressed genes (DEGs) were obtained comparing fruit stored at 4 °C with their control at 20 °C, and then specific and common up and down-regulated DEGs of each cultivar were identified. Functional analysis of these DEGs identified similarities between the response of zucchini fruit to low temperature and other stresses, with an important number of GO terms related to biotic and abiotic stresses overrepresented in both cultivars. This study also revealed several molecular mechanisms that could be related to chilling tolerance, since they were up-regulated in cv. Natura (CI tolerant) or down-regulated in cv. Sinatra (CI sensitive). These mechanisms were mainly those related to carbohydrate and energy metabolism, transcription, signal transduction, and protein transport and degradation. Among DEGs belonging to these pathways, we selected candidate genes that could regulate or promote chilling tolerance in zucchini fruit including the transcription factors MYB76-like, ZAT10-like, DELLA protein GAIP, and AP2/ERF domain-containing protein.

conclusionsThis study provides a broader understanding of the important mechanisms and processes related to coping with low temperature stress in zucchini fruit and allowed the identification of some candidate genes that may be involved in the acquisition of chilling tolerance in this crop. These genes will be the basis of future studies aimed to identify markers involved in cold tolerance and aid in zucchini breeding programs.

Indexed as

Cold TemperaturePreservation, BiologicalTranscriptomeAdaptation, PhysiologicalComputational BiologyCucurbitaEnergy MetabolismFruitGene Expression ProfilingGene Expression Regulation, PlantGene OntologyMolecular Sequence AnnotationCold tolerancePostharvest physiologyStress responseTranscriptomic profilingZucchini fruit

Identifiers

PMID29415652
PMCPMC5804050
OpenAlexW2799460607

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.