ArticlePloS one2018
Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- From genome to lifestyle: adaptive strategies ofApplied and environmental microbiology · 2026Article
- Genomic Insights into Phosphorus Solubilization ofMicroorganisms · 2025Article
- Salmonella enteritidis acquires phage resistance through a point mutation in rfbD but loses some of its environmental adaptability.Veterinary research · 2024Article
- Cold-active microbial enzymes and their biotechnological applications.Microbial biotechnology · 2024Review
- Cold adaptedFrontiers in microbiology · 2023Review
- Insights into the temperature responses of Pseudomonas species in beneficial and pathogenic host interactions.Applied microbiology and biotechnology · 2022Review
- Snowball Earth, population bottleneck andProceedings. Biological sciences · 2021Article
- Psychrophiles: A journey of hope.Journal of biosciences · 2021Review
- Pigment production by cold-adapted bacteria and fungi: colorful tale of cryosphere with wide range applications.Extremophiles : life under extreme conditions · 2020Review
- Quantitative Analysis of Cold Stress Inducing Lipidomic Changes inMolecules (Basel, Switzerland) · 2019Article
- Effect of copper on diesel degradation in Pseudomonas extremaustralis.Extremophiles : life under extreme conditions · 2019Article
- Lipid A structural characterization from the LPS of the Siberian psychro-tolerant Psychrobacter arcticus 273-4 grown at low temperature.Extremophiles : life under extreme conditions · 2018Article
- Reporting Key Features in Cold-Adapted Bacteria.Life (Basel, Switzerland) · 2018Review
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7 authors.
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Abstract
Psychrotroph microorganisms have developed cellular mechanisms to cope with cold stress. Cell envelopes are key components for bacterial survival. Outer membrane is a constituent of Gram negative bacterial envelopes, consisting of several components, such as lipopolysaccharides (LPS). In this work we investigated the relevance of envelope characteristics for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis by analyzing a mini Tn5 wapH mutant strain, encoding a core LPS glycosyltransferase. Our results showed that wapH strain is impaired to grow under low temperature but not for cold survival. The mutation in wapH, provoked a strong aggregative phenotype and modifications of envelope nanomechanical properties such as lower flexibility and higher turgor pressure, cell permeability and surface area to volume ratio (S/V). Changes in these characteristics were also observed in the wild type strain grown at different temperatures, showing higher cell flexibility but lower turgor pressure under cold conditions. Cold shock experiments indicated that an acclimation period in the wild type is necessary for cell flexibility and S/V ratio adjustments. Alteration in cell-cell interaction capabilities was observed in wapH strain. Mixed cells of wild type and wapH strains, as well as those of the wild type strain grown at different temperatures, showed a mosaic pattern of aggregation. These results indicate that wapH mutation provoked marked envelope alterations showing that LPS core conservation appears as a novel essential feature for active growth under cold conditions.
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