ArticlePloS one2018
gDNA extraction yield and methylation status of blood samples are affected by long-term storage conditions.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Hypertension and epigenetic aging: socio-biological pathways of health disparity.Clinical epigenetics · 2026Article
- Feasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS study.Scientific reports · 2025Article
- Epigenetic age and accelerated aging phenotypes: a tumor biomarker for predicting colorectal cancer.Aging · 2025Article
- DNA methylation in breast cancer: early detection and biomarker discovery through current and emerging approaches.Journal of translational medicine · 2025Review
- Biological Aging in Combination with Lifestyle Factors in the Blood-Based Methylome: A Biomarker for Colorectal Cancer Susceptibility in African American Women.Aging and disease · 2025Article
- Article
- Comment on "Genetic variants in miR-146a and miR-196a2 in endometriosis: a Brazilian study".Revista da Associacao Medica Brasileira (1992) · 2024Article
- Genome-wide methylation patterns from canine nanopore assemblies.G3 (Bethesda, Md.) · 2023Article
- Clinical significance of determining the hypermethylation of the RUNX3 gene promoter and its cohypermethylation with the BRCA1 gene for patients with breast cancer.Journal of cancer research and clinical oncology · 2023Article
- Degradation of methylation signals in cryopreserved DNA.Clinical epigenetics · 2023Article
- Parasite detection and quantification in avian blood is dependent on storage medium and duration.Ecology and evolution · 2023Article
- Utility evaluation of two molecular methods forHeliyon · 2023Article
- Methylation of promoter region of BRCA1 gene versus pathogenic variants of gene: risk factor or clinical marker of breast cancer.Breast cancer research and treatment · 2022Article
- Preanalytical Variables in the Analysis of Mitochondrial DNA in Whole Blood and Plasma from Pancreatic Cancer Patients.Diagnostics (Basel, Switzerland) · 2022Article
- Preserving avian blood and DNA sampled in the wild: A survey of personal experiences.Ecology and evolution · 2022Article
- Wild epigenetics: insights from epigenetic studies on natural populations.Proceedings. Biological sciences · 2022Review
- The Tiger Rattlesnake genome reveals a complex genotype underlying a simple venom phenotype.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Global DNA (hydroxy)methylation is stable over time under several storage conditions and temperatures.Epigenetics · 2021Article
- Calling Variants in the Clinic: Informed Variant Calling Decisions Based on Biological, Clinical, and Laboratory Variables.Computational and structural biotechnology journal · 2019Review
- Genome-wide study of the effect of blood collection tubes on the cell-free DNA methylome.EpigeneticsArticle
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epigenetics is believed to provide great chances for a better understanding of the development and treatment of many diseases where the analysis of genomic DNA has so far failed to provide conclusive answers. Methylcytosine is a frequently used quantitative marker of epigenetic studies. Since immediate analysis of sampled material is in most cases not possible, storage time and conditions are critical aspects regarding the quality of genomic DNA and reliability of analysis. Blood is frequently used for such analyses. We, therefore, collected blood samples of ten volunteers and stored them under various conditions for ten months: -70°C, -20°C, 2-8°C and room temperature. An additional aliquot was frozen at -70°C and thawed once a week at room temperature. We then compared the DNA extraction yields and methylation status in relation to storage time and conditions. We found significantly lower DNA extraction yields (up to -97.45%; p ≤ 0.001) as well as significantly higher methylation levels after ten months of storage (up to +42.0%; p ≤ 0.001). These results suggest that storage time has an important influence on DNA analyses of blood samples for all storage conditions. This might be due to differences in stability of methylated and non-methylated DNA. Our study indicates that storage conditions and time may be a critical factor for epigenetic methylation studies and require rigorous validation. For reliable analyses we, therefore, recommend to perform epigenetic analysis directly after sample collection.
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