ArticlePLoS genetics2018
Gli3 is a negative regulator of Tas1r3-expressing taste cells.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Expression ofbioRxiv : the preprint server for biology · 2026Article
- Developing and regenerating a sense of taste.Current topics in developmental biology · 2025Review
- Exploring the Role of Ccn3 in Type III Cell of Mice Taste Buds.Journal of neurochemistry · 2025Article
- Article
- Ascl1-expressing cell differentiation in initially developed taste buds and taste organoids.Cell and tissue research · 2023Article
- Anterior and Posterior Tongue Regions and Taste Papillae: Distinct Roles and Regulatory Mechanisms with an Emphasis on Hedgehog Signaling and Antagonism.International journal of molecular sciences · 2023Review
- Type II taste cells participate in mucosal immune surveillance.PLoS biology · 2023Article
- Role of Transient Receptor Potential Vanilloid 1 in Sonic Hedgehog-Dependent Taste Bud Differentiation.Life (Basel, Switzerland) · 2022Article
- The sense of taste: Development, regeneration, and dysfunction.WIREs mechanisms of disease · 2022Review
- Type II/III cell composition and NCAM expression in taste buds.Cell and tissue research · 2021Article
- Genetics of mouse behavioral and peripheral neural responses to sucrose.Mammalian genome : official journal of the International Mammalian Genome Society · 2021Article
- Chemosensory Dysfunction in Patients with COVID-19: What Do We Learn from the Global Outbreak?Current allergy and asthma reports · 2021Review
- A Mechanistic Overview of Taste Bud Maintenance and Impairment in Cancer Therapies.Chemical senses · 2021Review
- Consequences of Obesity on the Sense of Taste: Taste Buds as Treatment Targets?Diabetes & metabolism journal · 2020Review
- Review
- Studies of human twins reveal genetic variation that affects dietary fat perception.Chemical senses · 2020Article
- Single Cell Gene Co-Expression Network Reveals FECH/CROT Signature as a Prognostic Marker.Cells · 2019Article
- Hedgehog Signaling Regulates Taste Organs and Oral Sensation: Distinctive Roles in the Epithelium, Stroma, and Innervation.International journal of molecular sciences · 2019Review
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Mouse taste receptor cells survive from 3-24 days, necessitating their regeneration throughout adulthood. In anterior tongue, sonic hedgehog (SHH), released by a subpopulation of basal taste cells, regulates transcription factors Gli2 and Gli3 in stem cells to control taste cell regeneration. Using single-cell RNA-Seq we found that Gli3 is highly expressed in Tas1r3-expressing taste receptor cells and Lgr5+ taste stem cells in posterior tongue. By PCR and immunohistochemistry we found that Gli3 was expressed in taste buds in all taste fields. Conditional knockout mice lacking Gli3 in the posterior tongue (Gli3CKO) had larger taste buds containing more taste cells than did control wild-type (Gli3WT) mice. In comparison to wild-type mice, Gli3CKO mice had more Lgr5+ and Tas1r3+ cells, but fewer type III cells. Similar changes were observed ex vivo in Gli3CKO taste organoids cultured from Lgr5+ taste stem cells. Further, the expression of several taste marker and Gli3 target genes was altered in Gli3CKO mice and/or organoids. Mirroring these changes, Gli3CKO mice had increased lick responses to sweet and umami stimuli, decreased lick responses to bitter and sour taste stimuli, and increased glossopharyngeal taste nerve responses to sweet and bitter compounds. Our results indicate that Gli3 is a suppressor of stem cell proliferation that affects the number and function of mature taste cells, especially Tas1r3+ cells, in adult posterior tongue. Our findings shed light on the role of the Shh pathway in adult taste cell regeneration and may help devise strategies for treating taste distortions from chemotherapy and aging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.