ArticleRedox biology2018
A glutaredoxin in the mitochondrial intermembrane space has stage-specific functions in the thermo-tolerance and proliferation of African trypanosomes.
Article in Redox biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Loss of TGME49_227100 (Glutaredoxin 5) Disrupts Oocyst Formation and Sporulation inPathogens (Basel, Switzerland) · 2026Article
- Regulation of Mitochondrial Energy Metabolism by Glutaredoxin 5 in the Apicomplexan Parasite Neospora caninum.Microbiology spectrum · 2023Article
- Omics data integration facilitates target selection for new antiparasitic drugs against TriTryp infections.Frontiers in pharmacology · 2023Article
- Identification and Function of Apicoplast Glutaredoxins inInternational journal of molecular sciences · 2021Article
- Cloning, Expression, Characterization, and Antioxidant Protection of Glutaredoxin3 From Psychrophilic BacteriumFrontiers in microbiology · 2021Article
- The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes.Redox biology · 2020Article
- A tryparedoxin-coupled biosensor reveals a mitochondrial trypanothione metabolism in trypanosomes.eLife · 2020Article
- Glutaredoxin 1 Deficiency Leads to Microneme Protein-Mediated Growth Defects inFrontiers in microbiology · 2020Article
- An essential thioredoxin-type protein of Trypanosoma brucei acts as redox-regulated mitochondrial chaperone.PLoS pathogens · 2019Article
- Article
- Kinetic studies reveal a key role of a redox-active glutaredoxin in the evolution of the thiol-redox metabolism of trypanosomatid parasites.The Journal of biological chemistry · 2019Article
- The lineage-specific, intrinsically disordered N-terminal extension of monothiol glutaredoxin 1 from trypanosomes contains a regulatory region.Scientific reports · 2018Article
- Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypanosoma brucei glutaredoxin 2 (Grx2) is a dithiol glutaredoxin that is specifically located in the mitochondrial intermembrane space. Bloodstream form parasites lacking Grx2 or both, Grx2 and the cytosolic Grx1, are viable in vitro and infectious to mice suggesting that neither oxidoreductase is needed for survival or infectivity to mammals. A 37 °C to 39 °C shift changes the cellular redox milieu of bloodstream cells to more oxidizing conditions and induces a significantly stronger growth arrest in wildtype parasites compared to the mutant cells. Grx2-deficient cells ectopically expressing the wildtype form of Grx2 with its C31QFC34 active site, but not the C34S mutant, regain the sensitivity of the parental strain, indicating that the physiological role of Grx2 requires both active site cysteines. In the procyclic insect stage of the parasite, Grx2 is essential. Both alleles can be replaced if procyclic cells ectopically express authentic or C34S, but not C31S/C34S Grx2, pointing to a redox role that relies on a monothiol mechanism. RNA-interference against Grx2 causes a virtually irreversible proliferation defect. The cells adopt an elongated morphology but do not show any significant alteration in the cell cycle. The growth retardation is attenuated by high glucose concentrations. Under these conditions, procyclic cells obtain ATP by substrate level phosphorylation suggesting that Grx2 might regulate a respiratory chain component.
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