ArticleSLAS technology2018
Quantitative Live-Cell Confocal Imaging of 3D Spheroids in a High-Throughput Format.
Article in SLAS technology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Four-Dimensional Electrochemiluminescence Microscopy Enables Depth-Resolved Imaging of Single, Living Spheroids.Angewandte Chemie (International ed. in English) · 2026Article
- Three-dimensional spheroid models in breast cancer: tumor microenvironment complexity, cancer stem cell-driven resistance, and translational model integration.Journal of translational medicine · 2026Review
- Experimental workflow to evaluate nanoparticles combined with photon and carbon-ion medical irradiations in spheroids.Discover nano · 2026Article
- Role of atomic force microscopy in characterization of heterotypic cancer spheroids and their interaction with microplastic particles.Scientific reports · 2026Article
- Review
- A multi-stage weakly supervised design for spheroid segmentation to explore mesenchymal stem cell differentiation dynamics.BMC bioinformatics · 2025Article
- Parallel and High Throughput Reaction Monitoring with Computer Vision.Angewandte Chemie (International ed. in English) · 2025Article
- Cell viability imaging in tumor spheroidsChemical communications (Cambridge, England) · 2024Article
- Cost-effective optimized method to process 3D tumoral spheroids in microwell arrays for immunohistochemistry analysis.Journal of medicine and life · 2024Article
- Development of a human liver microphysiological coculture system for higher throughput chemical safety assessment.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Spheroids of FAP-Positive Cell Lines as a Model for Screening Drugs That Affect FAP Expression.Biomedicines · 2023Article
- Robust formation of optimal single spheroids towards cost-effective in vitro three-dimensional tumor models.FEBS open bio · 2023Article
- On-chip clearing for live imaging of 3D cell cultures.Biomedical optics express · 2023Article
- Three-Dimensional Spheroids for Cancer Research.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Gastric cancer multicellular spheroid analysis by two-photon microscopy.Biomedical optics express · 2022Article
- 3D Microtissues Mimic the Architecture, Estradiol Synthesis, and Gap Junction Intercellular Communication of the Avascular Granulosa.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- One-step visualization of natural cell activities in non-labeled living spheroids.Scientific reports · 2022Article
- Lipopolysaccharide-induced neuroinflammation disrupts functional connectivity and community structure in primary cortical microtissues.Scientific reports · 2021Article
- Os(II)-Bridged Polyarginine Conjugates: The Additive Effects of Peptides in Promoting or Preventing Permeation in Cells and Multicellular Tumor Spheroids.Inorganic chemistry · 2021Article
- Challenges of applying multicellular tumor spheroids in preclinical phase.Cancer cell international · 2021Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Accurately predicting the human response to new compounds is critical to a wide variety of industries. Standard screening pipelines (including both in vitro and in vivo models) often lack predictive power. Three-dimensional (3D) culture systems of human cells, a more physiologically relevant platform, could provide a high-throughput, automated means to test the efficacy and/or toxicity of novel substances. However, the challenge of obtaining high-magnification, confocal z stacks of 3D spheroids and understanding their respective quantitative limitations must be overcome first. To address this challenge, we developed a method to form spheroids of reproducible size at precise spatial locations across a 96-well plate. Spheroids of variable radii were labeled with four different fluorescent dyes and imaged with a high-throughput confocal microscope. 3D renderings of the spheroid had a complex bowl-like appearance. We systematically analyzed these confocal z stacks to determine the depth of imaging and the effect of spheroid size and dyes on quantitation. Furthermore, we have shown that this loss of fluorescence can be addressed through the use of ratio imaging. Overall, understanding both the limitations of confocal imaging and the tools to correct for these limits is critical for developing accurate quantitative assays using 3D spheroids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.