Evidence map›Paper›PMID 29412740›Full record

ArticleCellular reprogramming2018

Measles Virus Persistent Infection of Human Induced Pluripotent Stem Cells.

Hila Naaman, Tatiana Rabinski, Avi Yizhak, Solly Mizrahi, Yonat Shemer Avni, Ran Taube, Bracha Rager, Yacov Weinstein, Glenn Rall, Jacob Gopas and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular reprogramming, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. miRNA-mediated control of exogenousMolecular therapy. Methods & clinical development · 2022
    Article
  4. Article
  5. International journal of molecular sciences · 2018
    Article
  6. Molecular Mode of Action ofInternational journal of molecular sciences · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Hila Naaman1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
Tatiana Rabinski1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
Avi Yizhak3 Department of Surgery A, Soroka University Medical Center , Beer Sheva, Israel .
Solly Mizrahi3 Department of Surgery A, Soroka University Medical Center , Beer Sheva, Israel .
Yonat Shemer Avni1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
Ran Taube1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
Bracha Rager1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
Yacov Weinstein1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
Glenn Rall5 Fox Chase Cancer Center , Blood Cell Development and Function, Philadelphia, Pennsylvania, USA.
Jacob Gopas1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
Rivka Ofir1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .

Funding

The Role of Host-Encoded microRNAs in Maintaining Measles Virus PersistenceR03AI094164 · NIAID · RESEARCH INST OF FOX CHASE CAN CTR · PI RALL, GLENN F · 2012 to 2013
$143k
NIAID NIH HHS R03 AI094164
6 · The paper itself

Abstract

In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as receptors. It is not yet known how early in the embryonal differentiation stages these receptors are expressed. We established two hiPSCs (BGU-iPSCs and EMF-iPSCs) which express CD46 and CD150. Both cell types can be infected by MV to form persistent, noncytopathic cell lines that release infectious MV particles. Following MV persistent infection, BGU-iPSCs and EMF-iPSCs remain pluripotent and can differentiate in vitro into the three germ layers. This includes cells expressing the neuronal differentiation markers: NF68 and miRNA-124. Since the MV does not integrate into the cell's genome, it can be utilized as a vehicle to systematically introduce genes into iPSC, to dissect and to define factors regulating lineage differentiation.

Indexed as

AnimalsCell DifferentiationCell LineCell LineageGreen Fluorescent ProteinsHumansInduced Pluripotent Stem CellsMeasles virusMembrane Cofactor ProteinMiceMice, Inbred NODMice, SCIDMicroRNAsReceptors, VirusSignaling Lymphocytic Activation Molecule Family Member 1CD46 protein, humanGreen Fluorescent ProteinsMembrane Cofactor ProteinMicroRNAsMIRN124 microRNA, humanReceptors, VirusSignaling Lymphocytic Activation Molecule Family Member 1SLAMF1 protein, humanhuman-induced pluripotent stem cellsmeasles viruspersistent infection

Identifiers

PMID29412740
PMCPMC5804099

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.