Evidence map›Paper›PMID 29412179›Full record

ArticleBone2018

High-frequency spectral ultrasound imaging (SUSI) visualizes early post-traumatic heterotopic ossification (HO) in a mouse model.

Kavitha Ranganathan, Xiaowei Hong, David Cholok, Joe Habbouche, Caitlin Priest, Christopher Breuler, Michael Chung, John Li, Arminder Kaura, Hsiao Hsin Sung Hsieh and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bone, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Revisiting Heinz-Lippman disease as a complication of chronic venous insufficiency.Journal of vascular surgery cases and innovative techniques · 2024
    Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 1 institution in 1 country.

Kavitha RanganathanDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Xiaowei HongDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
David CholokDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Joe HabboucheDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Caitlin PriestDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Christopher BreulerDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Michael ChungDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
John LiDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Arminder KauraDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Hsiao Hsin Sung HsiehDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Jonathan ButtsDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Serra UcerDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Ean SchwartzDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Steven R BuchmanDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Jan P StegemannDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Cheri X DengDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.
Benjamin LeviDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA. Electronic address: blevi@med.umich.edu.
University of Michigan–Ann Arbor · US

Funding

TRAINING PROGRAM IN ORGANOGENESIST32HD007505 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PASCA DI MAGLIANO, MARINA, SPENCE, JASON · 1997 to 2021
$6.9M
Microscale Mechanobiology for Musculoskeletal Tissue Engineering using Advanced Ultrasound TechniquesR01DE026630 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DENG, CHERI X, STEGEMANN, JAN P. · 2017 to 2021
$1.8M
Targeting Molecular and Cellular Mediators of Inflammation to Prevent Pathologic Cell Differentiation and Heterotopic OssificationR01AR071379 · NIAMS · UT SOUTHWESTERN MEDICAL CENTER · PI LEVI, BENJAMIN · 2017 to 2021
$1.5M
Developing New Diagnostic and Timed, TAK1 Specific Treatment Strategies for Trauma Induced Heterotopic OssificationR01GM123069 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI LEVI, BENJAMIN · 2017 to 2020
$1.2M
Novel Pathway and Prevention Strategy for Heterotopic OssificationK08GM109105 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LEVI, BENJAMIN · 2014 to 2017
$785k
Targeting Heterotopic Ossification Induced Joint Contractures Before They Occur Through Early Detection and Angiogenic InhibitionF32AR068902 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI RANGANATHAN, KAVITHA · 2015 to 2016
$116k
NIAMS NIH HHS F32 AR068902NIAMS NIH HHS R01 AR071379NICHD NIH HHS T32 HD007505NIDCR NIH HHS R01 DE026630NIGMS NIH HHS K08 GM109105NIGMS NIH HHS R01 GM123069
6 · The paper itself

Abstract

purposeEarly treatment of heterotopic ossification (HO) is currently limited by delayed diagnosis due to limited visualization at early time points. In this study, we validate the use of spectral ultrasound imaging (SUSI) in an animal model to detect HO as early as one week after burn tenotomy.

methodsConcurrent SUSI, micro CT, and histology at 1, 2, 4, and 9weeks post-injury were used to follow the progression of HO after an Achilles tenotomy and 30% total body surface area burn (n=3-5 limbs per time point). To compare the use of SUSI in different types of injury models, mice (n=5 per group) underwent either burn/tenotomy or skin incision injury and were imaged using a 55MHz probe on VisualSonics VEVO 770 system at one week post injury to evaluate the ability of SUSI to distinguish between edema and HO. Average acoustic concentration (AAC) and average scatterer diameter (ASD) were calculated for each ultrasound image frame. Micro CT was used to calculate the total volume of HO. Histology was used to confirm bone formation.

resultsUsing SUSI, HO was visualized as early as 1week after injury. HO was visualized earliest by 4weeks after injury by micro CT. The average acoustic concentration of HO was 33% more than that of the control limb (n=5). Spectroscopic foci of HO present at 1week that persisted throughout all time points correlated with the HO present at 9weeks on micro CT imaging.

conclusionSUSI visualizes HO as early as one week after injury in an animal model. SUSI represents a new imaging modality with promise for early diagnosis of HO.

Indexed as

AnimalsDisease Models, AnimalEarly DiagnosisMiceOssification, HeterotopicOsteogenesisTenotomyUltrasonographyAchilles tenotomyBurn injuryHeterotopic ossificationMicro CTQuantitative ultrasoundSpectral ultrasound imaging

Identifiers

PMID29412179
PMCPMC5955392
OpenAlexW2790896417

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.