ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2018
Gene expression profiles of the meniscus avascular phenotype: A guide for meniscus tissue engineering.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Meniscus gene expression profiling of inner and outer zone meniscus tissue compared to cartilage and passaged monolayer meniscus cells.Scientific reports · 2024Article
- Preliminary identification of somatic mutations profile in ACL injury.Scientific reports · 2024Article
- "Slow walk" mimetic tensile loading maintains human meniscus tissue resident progenitor cells homeostasis in photocrosslinked gelatin hydrogel.Bioactive materials · 2023Article
- Formulation and evaluation of a bioink composed of alginate, gelatin, and nanocellulose for meniscal tissue engineering.International journal of bioprinting · 2023Article
- Histologic and molecular features in pathologic human menisci from knees with and without osteoarthritis.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Collagen fibrous scaffolds for sustained delivery of growth factors for meniscal tissue engineering.Nanomedicine (London, England) · 2022Article
- Pneumatospinning Biomimetic Scaffolds for Meniscus Tissue Engineering.Frontiers in bioengineering and biotechnology · 2022Article
- Meniscus cell regional phenotypes: Dedifferentiation and reversal by biomaterial embedding.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Three-dimensional (3D) hydrogel serves as a platform to identify potential markers of chondrocyte dedifferentiation by combining RNA sequencing.Bioactive materials · 2021Article
- Assessing the Resident Progenitor Cell Population and the Vascularity of the Adult Human Meniscus.Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association · 2021Article
- Implementation of Endogenous and Exogenous Mesenchymal Progenitor Cells for Skeletal Tissue Regeneration and Repair.Bioengineering (Basel, Switzerland) · 2020Review
- Pharmacological blockade of PCAF ameliorates osteoarthritis development via dual inhibition of TNF-α-driven inflammation and ER stress.EBioMedicine · 2019Article
- Core-Shell Nanofibrous Scaffolds for Repair of Meniscus Tears.Tissue engineering. Part A · 2019Article
- Human engineered meniscus transcriptome after short-term combined hypoxia and dynamic compression.Journal of tissue engineeringArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Avascular (Avas) meniscus regeneration remains a challenge, which is partly a consequence of our limited knowledge of the cells that maintain this tissue region. In this study, we utilized microarrays to characterize gene expression profiles of intact human Avas meniscus tissue and of cells following culture expansion. Using these data, we examined various 3D culture conditions to redifferentiate Avas cells toward the tissue phenotype. RNA was isolated from either the tissue directly or following cell isolation and 2 weeks in monolayer culture. RNA was hybridized on human genome arrays. Differentially expressed (DE) genes were identified by ranking analysis. DAVID pathway analysis was performed and visualized using STRING analysis. Quantitative PCR (qPCR) on additional donor menisci (tissues and cells) were used to validate array data. Avas cells cultured in 3D were subjected to qPCR to compare with the array-generated data. A total of 387 genes were DE based on differentiation state (>3-fold change; p < 0.01). In Avas-cultured cells, the upregulated pathways included focal adhesion, ECM-receptor interaction, regulation of actin cytoskeleton, and PDGF Signaling. In 3D-cultured Avas cells, TGFβ1 or combinations of TGFβ1 and BMP6 were most effective to promote an Avas tissue phenotype. THBS2 and THBS4 expression levels were identified as a means to denote meniscus cell phenotype status. We identified the key gene expression profiles, new markers and pathways involved in characterizing the Avas meniscus phenotype in the native state and during in vitro dedifferentiation and redifferentiation. These data served to screen 3D conditions to generate meniscus-like neotissues. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1947-1958, 2018.
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