Evidence map›Paper›PMID 29411671›Full record

ArticleAdipocyte2018

Mirna biogenesis pathway is differentially regulated during adipose derived stromal/stem cell differentiation.

E C Martin, A T Qureshi, C B Llamas, M E Burow, A G King, O C Lee, V Dasa, M A Freitas, J A Forsberg, E A Elster and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Adipocyte, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Downregulation ofInternational journal of molecular sciences · 2023
    Article
  5. Article
  6. Article
  7. Article
  8. Altered Regulation of adipomiR Editing with Aging.International journal of molecular sciences · 2020
    Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 1 country.

E C Martina Department for Biological and Agricultural Engineering , Louisiana State University , Baton Rouge , LA.
A T Qureshib Department of Regenerative Medicine , Naval Medical Research Center , Silver Spring , MD , USA.
C B Llamash Center for Regenerative Medicine and Stem Cell Therapy, Tulane University School of Medicine , New Orleans , LA , USA.
M E Burowi Department of Hematology/Oncology , Tulane University School of Medicine , New Orleans , LA , USA.
A G Kingc Department of Orthopedics , Louisiana State University Health Science Center , New Orleans , LA , USA.
O C Leec Department of Orthopedics , Louisiana State University Health Science Center , New Orleans , LA , USA.
V Dasac Department of Orthopedics , Louisiana State University Health Science Center , New Orleans , LA , USA.
M A Freitasd Department of Molecular Virology , Immunology Medical & Genetics, Ohio State University , Columbus , OH , USA.
J A Forsberge Department of Surgery , Uniformed Services University of the Health Sciences-Walter Reed National Military Medical Center Bethesda , MD , USA.
E A Elsterb Department of Regenerative Medicine , Naval Medical Research Center , Silver Spring , MD , USA.
T A Davisb Department of Regenerative Medicine , Naval Medical Research Center , Silver Spring , MD , USA.
J M Gimblef Departments of Medicine , Structural and Cellular Biology, & Surgery, Tulane University School of Medicine , New Orleans , LA , USA.
Louisiana State University Health Sciences Center New Orleans · USNaval Medical Research Command · USTulane University · USLouisiana State University · USThe Ohio State University · USUniformed Services University of the Health Sciences · US

Funding

Tracking & Evaluation CoreU54GM104940 · NIGMS · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · PI Peter Todd Katzmarzyk · 2012 to 2026
$69.1M
NIGMS NIH HHS U54 GM104940
6 · The paper itself

Abstract

Stromal/stem cell differentiation is controlled by a vast array of regulatory mechanisms. Included within these are methods of mRNA gene regulation that occur at the level of epigenetic, transcriptional, and/or posttranscriptional modifications. Current studies that evaluate the posttranscriptional regulation of mRNA demonstrate microRNAs (miRNAs) as key mediators of stem cell differentiation through the inhibition of mRNA translation. miRNA expression is enhanced during both adipogenic and osteogenic differentiation; however, the mechanism by which miRNA expression is altered during stem cell differentiation is less understood. Here we demonstrate for the first time that adipose-derived stromal/stem cells (ASCs) induced to an adipogenic or osteogenic lineage have differences in strand preference (-3p and -5p) for miRNAs originating from the same primary transcript. Furthermore, evaluation of miRNA expression in ASCs demonstrates alterations in both miRNA strand preference and 5'seed site heterogeneity. Additionally, we show that during stem cell differentiation there are alterations in expression of genes associated with the miRNA biogenesis pathway. Quantitative RT-PCR demonstrated changes in the Argonautes (AGO1-4), Drosha, and Dicer at intervals of ASC adipogenic and osteogenic differentiation compared to untreated ASCs. Specifically, we demonstrated altered expression of the AGOs occurring during both adipogenesis and osteogenesis, with osteogenesis increasing AGO1-4 expression and adipogenesis decreasing AGO1 gene and protein expression. These data demonstrate changes to components of the miRNA biogenesis pathway during stromal/stem cell differentiation. Identifying regulatory mechanisms for miRNA processing during ASC differentiation may lead to novel mechanisms for the manipulation of lineage differentiation of the ASC through the global regulation of miRNA as opposed to singular regulatory mechanisms.

Indexed as

Cell DifferentiationAdipose TissueCells, CulturedHumansMesenchymal Stem CellsMicroRNAsMicroRNAsbiogenesismiRNAstem cell differentiation

Identifiers

PMID29411671
PMCPMC6152510
OpenAlexW2782725032

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.