ArticleNature communications2018
Biochemical mechanisms determine the functional compatibility of heterologous genes.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Machine learning enables prediction of metabolic system evolution in bacteria.Science advances · 2023Pooled it
- Highly Resolved Community Sewage Metagenomics Unveiling Landscape and Transmission Patterns of Antibiotic Resistome in Hong Kong Populations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Variations in carbapenem resistance associated with the VIM-1 metallo-β-lactamase across theMicrobiology (Reading, England) · 2026Article
- Genetic compatibility and ecological connectivity drive the dissemination of antibiotic resistance genes.Nature communications · 2025Article
- Current state and future prospects of Horizontal Gene Transfer detection.NAR genomics and bioinformatics · 2025Review
- Costs of antibiotic resistance genes depend on host strain and environment and can influence community composition.Proceedings. Biological sciences · 2024Article
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- Noise reduction strategies in metagenomic chromosome confirmation capture to link antibiotic resistance genes to microbial hosts.Microbial genomics · 2023Article
- Characterization of antibiotic resistomes by reprogrammed bacteriophage-enabled functional metagenomics in clinical strains.Nature microbiology · 2023Article
- Bacterial plasmid-associated and chromosomal proteins have fundamentally different properties in protein interaction networks.Scientific reports · 2022Article
- Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host.eLife · 2022Article
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- Introduction: the secret lives of microbial mobile genetic elements.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Article
- Evolutionary Pathways and Trajectories in Antibiotic Resistance.Clinical microbiology reviews · 2021Article
- Phylum barrier andMicrobial genomics · 2021Article
- Beyond horizontal gene transfer: the role of plasmids in bacterial evolution.Nature reviews. Microbiology · 2021Review
- Forecasting the dissemination of antibiotic resistance genes across bacterial genomes.Nature communications · 2021Article
- Insight of transcriptional regulators reveals the tolerance mechanism of carpet-grass (Axonopus compressus) against drought.BMC plant biology · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elucidating the factors governing the functional compatibility of horizontally transferred genes is important to understand bacterial evolution, including the emergence and spread of antibiotic resistance, and to successfully engineer biological systems. In silico efforts and work using single-gene libraries have suggested that sequence composition is a strong barrier for the successful integration of heterologous genes. Here we sample 200 diverse genes, representing >80% of sequenced antibiotic resistance genes, to interrogate the factors governing genetic compatibility in new hosts. In contrast to previous work, we find that GC content, codon usage, and mRNA-folding energy are of minor importance for the compatibility of mechanistically diverse gene products at moderate expression. Instead, we identify the phylogenetic origin, and the dependence of a resistance mechanism on host physiology, as major factors governing the functionality and fitness of antibiotic resistance genes. These findings emphasize the importance of biochemical mechanism for heterologous gene compatibility, and suggest physiological constraints as a pivotal feature orienting the evolution of antibiotic resistance.
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