Evidence map›Paper›PMID 29409958›Full record

ArticleProtein expression and purification2018

Expression, purification, and electrophysiological characterization of a recombinant, fluorescent Kir6.2 in mammalian cells.

Mark T Agasid, Xuemin Wang, Yiding Huang, Colleen M Janczak, Robert Bränström, S Scott Saavedra, Craig A Aspinwall

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein expression and purification, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Mark T AgasidDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, United States.
Xuemin WangDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, United States.
Yiding HuangDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, United States.
Colleen M JanczakDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, United States.
Robert BränströmDepartment of Molecular Medicine and Surgery, Karolinksa Institutet, Stockholm, Sweden.
S Scott SaavedraDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, United States; BIO5 Institute, University of Arizona, Tucson, AZ 85721, United States. Electronic address: saavedra@email.arizona.edu.
Craig A AspinwallDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, United States; BIO5 Institute, University of Arizona, Tucson, AZ 85721, United States; Department of Biomedical Engineering, University of Arizona, Tucson, AZ 85721, United States. Electronic address: aspinwal@email.arizona.edu.
University of Arizona · USKarolinska Institutet · SE

Funding

VITAMIN AP30CA023074 · NCI · UNIVERSITY OF ARIZONA · PI Dan Theodorescu · 1985 to 2026
$110.2M
Sniffer Biosensors Based on Ion Channel-GPCR Chimeras and Polylipid MembranesR01EB007047 · NIBIB · UNIVERSITY OF ARIZONA · PI ASPINWALL, CRAIG A, SAAVEDRA, STEVEN SCOTT · 2006 to 2015
$3.5M
Bio/Chemical Mapping of Pancreatic Cell ActivityR21EB022297 · NIBIB · TRUSTEES OF INDIANA UNIVERSITY · PI BAKER, LANE A. · 2016 to 2017
$407k
Radiation and Fluorescence Imaging Technology for Biology and BiochemistryS10OD013237 · OD · UNIVERSITY OF ARIZONA · PI PARK, CHAD KING · 2013 to 2013
$137k
NCI NIH HHS P30 CA023074NIBIB NIH HHS R01 EB007047NIBIB NIH HHS R21 EB022297NIH HHS S10 OD013237
6 · The paper itself

Abstract

The inwardly rectifying K

Indexed as

Adenosine TriphosphateAmino Acid SequenceChromatography, AffinityChromatography, GelGene ExpressionGreen Fluorescent ProteinsHEK293 CellsHumansKcnj11 ChannelLipid BilayersPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingRecombinant Fusion ProteinsSequence DeletionSolubilityTransfectionAdenosine Triphosphateenhanced green fluorescent proteinGreen Fluorescent ProteinsKcnj11 ChannelLipid BilayersPotassium Channels, Inwardly RectifyingRecombinant Fusion ProteinsBlack lipid membraneElectrophysiologyKir6.2Recombinant ion channelReconstitution

Identifiers

PMID29409958
PMCPMC5864551
OpenAlexW2794249282

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.