ArticlePloS one2018
Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Reverse vaccinology for development of vaccine against Aeromonas hydrophila infection in nile tilapia (Oreochromis niloticus).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Microalgal colony blot: A simple and rapid method for direct detection of recombinant protein production in microalgae colonies.PloS one · 2026Article
- Efficient secretion of a plastic degrading enzyme from the green algae Chlamydomonas reinhardtii.Scientific reports · 2025Article
- Engineering microalgal cell wall-anchored proteins using GP1 PPSPX motifs and releasing with intein-mediated fusion.bioRxiv : the preprint server for biology · 2025Article
- Effect of recombinant protein production and release on microalgal fitness and the impact of environmental conditions for localized therapeutic delivery.Journal of biological engineering · 2025Article
- Identification and Overexpression of Endogenous Transcription Factors to Enhance Lipid Accumulation in the Commercially Relevant SpeciesbioRxiv : the preprint server for biology · 2025Article
- Internalization of affinity tags enables the purification of secreted Chlamydomonas proteins.Current genetics · 2025Article
- Engineering the Novel Extremophile AlgaACS ES&T engineering · 2025Article
- Engineering the green algae Chlamydomonas incerta for recombinant protein production.PloS one · 2025Article
- Efficient secretory production of recombinant proteins in microalgae using an exogenous signal peptide.Frontiers in microbiology · 2025Article
- Fine-tuning the N-glycosylation of recombinant human erythropoietin using Chlamydomonas reinhardtii mutants.Plant biotechnology journal · 2024Article
- Establishing the green algaebioRxiv : the preprint server for biology · 2024Article
- Proteomic analysis reveals molecular changes following genetic engineering in Chlamydomonas reinhardtii.BMC microbiology · 2024Article
- Bioinformatic Prediction and High Throughput In Vivo Screening to Identify Cis-Regulatory Elements for the Development of Algal Synthetic Promoters.ACS synthetic biology · 2024Article
- Comparing the Ability of Secretory Signal Peptides for Heterologous Expression of Anti-Lipopolysaccharide Factor 3 inMarine drugs · 2023Article
- Secretory Expression and Application of Antilipopolysaccharide Factor 3 inBioengineering (Basel, Switzerland) · 2023Article
- Towards microalga-based superfoods: heterologous expression of zeolin inFrontiers in plant science · 2023Article
- Unassembled cell wall proteins form aggregates in the extracellular space of Chlamydomonas reinhardtii strain UVM4.Applied microbiology and biotechnology · 2022Article
- Production, Processing, and Protection of Microalgal n-3 PUFA-Rich Oil.Foods (Basel, Switzerland) · 2022Review
- Advances in Biosynthesis of Natural Products from Marine Microorganisms.Microorganisms · 2021Review
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Authors and funding
3 authors.
Funding
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Abstract
Efficient protein secretion is a desirable trait for any recombinant protein expression system, together with simple, low-cost, and defined media, such as the typical media used for photosynthetic cultures of microalgae. However, low titers of secreted heterologous proteins are usually obtained, even with the most extensively studied microalga Chlamydomonas reinhardtii, preventing their industrial application. In this study, we aimed to expand and evaluate secretory signal peptides (SP) for heterologous protein secretion in C. reinhardtii by comparing previously described SP with untested sequences. We compared the SPs from arylsulfatase 1 and carbonic anhydrase 1, with those of untried SPs from binding protein 1, an ice-binding protein, and six sequences identified in silico. We identified over 2000 unique SPs using the SignalP 4.0 software. mCherry fluorescence was used to compare the protein secretion of up to 96 colonies for each construct, non-secretion construct, and parental wild-type cc1690 cells. Supernatant fluorescence varied according to the SP used, with a 10-fold difference observed between the highest and lowest secretors. Moreover, two SPs identified in silico secreted the highest amount of mCherry. Our results demonstrate that the SP should be carefully selected and that efficient sequences can be coded in the C. reinhardtii genome. The SPs described here expand the portfolio available for research on heterologous protein secretion and for biomanufacturing applications.
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