Evidence map›Paper›PMID 29408716›Full record

ArticleWorld neurosurgery2018

Scanning Fiber Endoscope Improves Detection of 5-Aminolevulinic Acid-Induced Protoporphyrin IX Fluorescence at the Boundary of Infiltrative Glioma.

Evgenii Belykh, Eric J Miller, Danying Hu, Nikolay L Martirosyan, Eric C Woolf, Adrienne C Scheck, Vadim A Byvaltsev, Peter Nakaji, Leonard Y Nelson, Eric J Seibel and 1 more

Open access · greenAbstract readComparative StudyEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in World neurosurgery, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 1 pooled it
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.

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  17. Characterization ofFrontiers in oncology · 2022
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Evgenii BelykhDepartment of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, USA; Department of Neurosurgery, Irkutsk State Medical University, Irkutsk, Russia.
Eric J MillerDepartment of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, USA.
Danying HuBiorobotics Laboratory, Department of Electrical Engineering, University of Washington, Seattle, Washington, USA.
Nikolay L MartirosyanDepartment of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, USA.
Eric C WoolfDepartment of Neuro-Oncology Research, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, USA.
Adrienne C ScheckDepartment of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, USA; Department of Neuro-Oncology Research, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, USA.
Vadim A ByvaltsevDepartment of Neurosurgery, Irkutsk State Medical University, Irkutsk, Russia.
Peter NakajiDepartment of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, USA.
Leonard Y NelsonHuman Photonics Laboratory, Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA.
Eric J SeibelHuman Photonics Laboratory, Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA.
Mark C PreulDepartment of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, USA. Electronic address: Neuropub@barrowneuro.org.
St. Joseph's Hospital and Medical Center · USBarrow Neurological Institute · USUniversity of Washington · USIrkutsk State Medical University · RU

Funding

NRI: Advanced Biophotonics for Image-guided Robotic SurgeryR01EB016457 · NIBIB · UNIVERSITY OF WASHINGTON · PI SEIBEL, ERIC J · 2012 to 2015
$1.2M
NIBIB NIH HHS R01 EB016457
6 · The paper itself

Abstract

objectiveFluorescence-guided surgery with protoporphyrin IX (PpIX) as a photodiagnostic marker is gaining acceptance for resection of malignant gliomas. Current wide-field imaging technologies do not have sufficient sensitivity to detect low PpIX concentrations. We evaluated a scanning fiber endoscope (SFE) for detection of PpIX fluorescence in gliomas and compared it to an operating microscope (OPMI) equipped with a fluorescence module and to a benchtop confocal laser scanning microscope (CLSM).

methods5-Aminolevulinic acid-induced PpIX fluorescence was assessed in GL261-Luc2 cells in vitro and in vivo after implantation in mouse brains, at an invading glioma growth stage, simulating residual tumor. Intraoperative fluorescence of high and low PpIX concentrations in normal brain and tumor regions with SFE, OPMI, CLSM, and histopathology were compared.

resultsSFE imaging of PpIX correlated to CLSM at the cellular level. PpIX accumulated in normal brain cells but significantly less than in glioma cells. SFE was more sensitive to accumulated PpIX in fluorescent brain areas than OPMI (P < 0.01) and dramatically increased imaging time (>6×) before tumor-to-background contrast was diminished because of photobleaching.

conclusionsSFE provides new endoscopic capabilities to view PpIX-fluorescing tumor regions at cellular resolution. SFE may allow accurate imaging of 5-aminolevulinic acid labeling of gliomas and other tumor types when current detection techniques have failed to provide reliable visualization. SFE was significantly more sensitive than OPMI to low PpIX concentrations, which is relevant to identifying the leading edge or metastasizing cells of malignant glioma or to treating low-grade gliomas. This new application has the potential to benefit surgical outcomes.

Indexed as

NeuroendoscopesAdministration, OralAminolevulinic AcidAnimalsBiotransformationBrain NeoplasmsCell Line, TumorFemaleFiber Optic TechnologyFluorescent DyesGenes, ReporterGliomaMiceMice, Inbred C57BLMicroscopy, ConfocalMicroscopy, FluorescenceAminolevulinic AcidFluorescent DyesPhotosensitizing Agentsprotoporphyrin IXProtoporphyrins5-Aminolevulinic acidFluorescence-guided surgeryGliomaMicroscopyProtoporphyrin IXScanning fiber endoscope

Identifiers

PMID29408716
PMCPMC5924630
OpenAlexW2791401693

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.