Evidence map›Paper›PMID 29408391›Full record

ArticleJournal of neuroscience methods2018

Single-injection ex ovo transplantation method for broad spinal cord engraftment of human pluripotent stem cell-derived motor neurons.

Maria C Estevez-Silva, Akshitha Sreeram, Stephanie Cuskey, Nikolai Fedorchak, Nisha Iyer, Randolph S Ashton

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroscience methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Maria C Estevez-SilvaWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, USA; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Akshitha SreeramWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, USA.
Stephanie CuskeyWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, USA.
Nikolai FedorchakWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, USA.
Nisha IyerWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, USA.
Randolph S AshtonWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, USA; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. Electronic address: rashton2@wisc.edu.
University of Wisconsin–Madison · USWisconsin Institutes for Discovery · US

Funding

High-Throughput Modeling of ALS Using iPSC-Derived Neural Tube MicroarraysR33NS082618 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI ASHTON, RANDOLPH S · 2017 to 2018
$779k
High-Throughput Modeling of ALS Using iPSC-Derived Neural Tube MicroarraysR21NS082618 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI ASHTON, RANDOLPH S · 2014 to 2015
$348k
NINDS NIH HHS R21 NS082618NINDS NIH HHS R33 NS082618
6 · The paper itself

Abstract

backgroundTransplantation of human pluripotent stem cell (hPSC)-derived neurons into chick embryos is an established preliminary assay to evaluate engraftment potential. Yet, with recent advances in deriving diverse human neuronal subtypes, optimizing and standardizing such transplantation methodology for specific subtypes at their correlated anatomical sites is still required. NEW

methodWe determined the optimal stage of hPSC-derived motor neuron (hMN) differentiation for ex ovo transplantation, and developed a single injection protocol that implants hMNs throughout the spinal cord enabling broad regional engraftment possibilities.

resultsA single injection into the neural tube lumen yielded a 100% chick embryo survival and successful transplantation rate with MN engraftment observed from the rostral cervical through caudal lumbar spinal cord. Transplantation of HB9 COMPARISON WITH EXISTING METHOD(S): Our single injection hMN transplant method is the first to standardize the optimal hMN phenotype for chick embryo transplantation, provide a rubric for engraftment quantification, and enable broad engraftment throughout the spinal cord with a single surgical intervention.

conclusionTransplantation of HB9

Indexed as

AnimalsCell LineChick EmbryoHumansModels, AnimalMotor NeuronsNeural TubeNeurogenesisPluripotent Stem CellsSpinal CordTransplantation, HeterologousChick embryosHOX genesHUES3 HB9:GFPRegional phenotypeXenotransplantation

Identifiers

PMID29408391
PMCPMC5835419
OpenAlexW2792545915

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.