Evidence map›Paper›PMID 29405961›Full record

ArticlePlacenta2018

Sirtuin1 is required for proper trophoblast differentiation and placental development in mice.

Kanaga Arul Nambi Rajan, Marwa Khater, Francesca Soncin, Donald Pizzo, Matteo Moretto-Zita, Jonathan Pham, Orysya Stus, Pooja Iyer, Veronique Tache, Louise C Laurent and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Placenta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 46 citations in OpenAlex.

  1. Article
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  8. Conserved and divergent features of trophoblast stem cells.Journal of molecular endocrinology · 2024
    Review
  9. Review
  10. Review
  11. SIRT1: A Novel Protective Molecule in Pre-eclampsia.International journal of medical sciences · 2022
    Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Kanaga Arul Nambi RajanDepartment of Pathology, University of California San Diego, La Jolla, CA, 92093, USA; Sanford Consortium for Regenerative Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Marwa KhaterSanford Consortium for Regenerative Medicine, University of California San Diego, La Jolla, CA, 92093, USA; Department of Reproductive Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Francesca SoncinDepartment of Pathology, University of California San Diego, La Jolla, CA, 92093, USA; Sanford Consortium for Regenerative Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Donald PizzoDepartment of Pathology, University of California San Diego, La Jolla, CA, 92093, USA.
Matteo Moretto-ZitaDepartment of Pathology, University of California San Diego, La Jolla, CA, 92093, USA; Sanford Consortium for Regenerative Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Jonathan PhamDepartment of Pathology, University of California San Diego, La Jolla, CA, 92093, USA; Sanford Consortium for Regenerative Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Orysya StusDepartment of Pathology, University of California San Diego, La Jolla, CA, 92093, USA; Sanford Consortium for Regenerative Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Pooja IyerDepartment of Pathology, University of California San Diego, La Jolla, CA, 92093, USA.
Veronique TacheDepartment of Obstetrics and Gynecology, University of California Davis, Sacramento, CA, 95817, USA.
Louise C LaurentSanford Consortium for Regenerative Medicine, University of California San Diego, La Jolla, CA, 92093, USA; Department of Reproductive Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Mana M ParastDepartment of Pathology, University of California San Diego, La Jolla, CA, 92093, USA; Sanford Consortium for Regenerative Medicine, University of California San Diego, La Jolla, CA, 92093, USA. Electronic address: mparast@ucsd.edu.
University of California San Diego · USUniversity of California, Davis · US

Funding

Sirt1-PPARgamma signaling in placental development and fetal growth disordersR01HD071100 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PARAST, MANA M · 2012 to 2016
$1.6M
NICHD NIH HHS R01 HD071100
6 · The paper itself

Abstract

introductionPlacental insufficiency, arising from abnormal trophoblast differentiation and function, is a major cause of fetal growth restriction. Sirtuin-1 (Sirt1) is a ubiquitously-expressed NAD-dependent protein deacetylase which plays a key role in numerous cellular processes, including cellular differentiation and metabolism. Though Sirt1 has been widely studied, its role in placentation and trophoblast differentiation is unclear.

methodSirt1-heterozygous mice were mated and evaluated at various points during embryogenesis. In situ hybridization and immunohistochemistry were used to further characterize the placental phenotype of Sirt1-null mice. Wild-type (WT) and Sirt1-null mouse trophoblast stem cell (TSC) lines were derived from e3.5 littermate blastocysts. These cells were then evaluated at various points following differentiation. Differentiation was evaluated by expression of lineage specific markers using qPCR and flow cytometry, as well as Matrigel invasion assays. Global gene expression changes were evaluated using microarray-based RNA profiling; changes in specific pathways were validated using qPCR and western blot.

resultsIn the absence of Sirt1, both embryos and placentas were small, with placentas showing abnormalities in both the labyrinthine layer and junctional zone. Sirt1-null TSCs exhibited an altered phenotype in both undifferentiated and differentiated states, phenotypes which corresponded to changes in pathways relevant to both TSC maintenance and differentiation. Specifically, Sirt1-null TSC showed blunted differentiation, and appeared to be suspended in an Epcam DISCUSSION: Our results suggest that Sirt1 is required for proper TSC differentiation and placental development.

Indexed as

AnimalsCell DifferentiationFemaleMiceMice, KnockoutPlacentaPlacentationPregnancySirtuin 1TrophoblastsSirtuin 1DifferentiationFetal developmentFetal growth restrictionPlacentaStem cellsTrophoblast

Identifiers

PMID29405961
PMCPMC5805473
OpenAlexW2772767379

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.