ArticleVirulence2018
O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli.
Article in Virulence, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Prevalence of bovine endometritis, virulence mechanisms ofVirulence · 2026Pooled it
- Non-specific Protein and Peptide Antibacterial Factors of Mammals.The protein journal · 2026Review
- Harnessing pro-inflammatory and immunopathologic immune responses in urinary tract infections for vaccine development: it's all about a balance.Frontiers in immunology · 2026Review
- The Bacterial Swiss Army Knife: ExPEC Utilizes Multiple Resistance Mechanisms to Counteract Host Immune Responses.Vaccines · 2025Review
- Reversible excision of the wzy locus in Salmonella Typhimurium may aid recovery following phage predation.PLoS genetics · 2025Article
- Synergy between Group 2 capsules and lipopolysaccharide underpins serum resistance in extra-intestinal pathogenicMicrobiology (Reading, England) · 2024Article
- Bacterial Virus Forcing of Bacterial O-Antigen Shields: Lessons from Coliphages.International journal of molecular sciences · 2023Review
- Genome-wide identification of genes critical forVirulence · 2023Article
- O-Polysaccharides of LPS ModulateMicroorganisms · 2023Article
- Platelets andBiomedicines · 2022Review
- Two EnteropathogenicJournal of microbiology and biotechnology · 2021Article
- Structure and genetics of Escherichia coli O antigens.FEMS microbiology reviews · 2020Review
- Lipopolysaccharide O-antigens-bacterial glycans made to measure.The Journal of biological chemistry · 2020Review
- Identification of Host Adaptation Genes in Extraintestinal Pathogenic Escherichia coli during Infection in Different Hosts.Infection and immunity · 2019Article
- Editorial: O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli.Virulence · 2018Article
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7 authors at 3 institutions in 2 countries.
Funding
Abstract
Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading cause of bloodstream and other extraintestinal infections in human and animals. The greatest challenge encountered by ExPEC during an infection is posed by the host defense mechanisms, including lysozyme. ExPEC have developed diverse strategies to overcome this challenge. The aim of this study was to characterize the molecular mechanism of ExPEC resistance to lysozyme. For this, 15,000 transposon mutants of a lysozyme-resistant ExPEC strain NMEC38 were screened; 20 genes were identified as involved in ExPEC resistance to lysozyme-of which five were located in the gene cluster between galF and gnd, and were further confirmed to be involved in O-specific polysaccharide biosynthesis. The O-specific polysaccharide was able to inhibit the hydrolytic activity of lysozyme; it was also required by the complete lipopolysaccharide (LPS)-mediated protection of ExPEC against the bactericidal activity of lysozyme. The O-specific polysaccharide was further shown to be able to directly interact with lysozyme. Furthermore, LPS from ExPEC strains of different O serotypes was also able to inhibit the hydrolytic activity of lysozyme. Because of their cell surface localization and wide distribution in Gram-negative bacteria, O-specific polysaccharides appear to play a long-overlooked role in protecting bacteria against exogenous lysozyme.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.