Evidence map›Paper›PMID 29404971›Full record

ArticleJournal of fluorescence2018

Detection of Single Quantum Dots in Model Systems with Sheet Illumination Microscopy.

Mike Friedrich, Revaz Nozadze, Sandra de Keijzer, Ralf Steinmeyer, Vladimir Ermolayev, Gregory S Harms

Abstract read
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In one paragraph

Article in Journal of fluorescence, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mike FriedrichMolecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080, Würzburg, Germany.
Revaz NozadzeMolecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080, Würzburg, Germany.
Sandra de KeijzerMolecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080, Würzburg, Germany.
Ralf SteinmeyerMolecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080, Würzburg, Germany.
Vladimir ErmolayevMolecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080, Würzburg, Germany.
Gregory S HarmsMolecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080, Würzburg, Germany. gregory.harms@imaging-microscopy.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single molecule detection and tracking provides at times the only possible method to observe the interactions of low numbers of biomolecules, inlcuding DNA, receptors and signal mediating proteins in living systems. However, most existing imaging methods do not enable both high sensitivity and non-invasive imaging of large specimens. In this study we report a new setup for selective plane illumination microscopy (SPIM), which enables fast imaging and single molecule tracking with the resolution of confocal microscopy and the optical penetration beyond 300 μm. We detect and report our instrumental figures of merit, control values of fluorescence properties of single nano crystals in comparison to both standard widefield configurations, and also values of nanocrystals in multicellular "fruiting bodies" of Dictyostelium, an excellent control as a model developmental system. In the Dictyostelium , we also report some of our first tracking of single nanocrystals with SPIM. The new SPIM setup represents a new technique, which enables fast single molecule imaging and tracking in living systems.

Indexed as

DictyosteliumQuantum dotsSelective plane illumination microscopySheet illumination microscopySingle-molecule tracking

Identifiers

PMID29404971

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.