Evidence map›Paper›PMID 29404863›Full record

ArticleJournal of assisted reproduction and genetics2018

In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration.

Lusine Aghajanova, Sahar Houshdaran, Shaina Balayan, Evelina Manvelyan, Juan C Irwin, Heather G Huddleston, Linda C Giudice

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed, 5 pooled it
8.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 5 syntheses or guidelines pooled it, 132 citations in OpenAlex.

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  5. Platelet-rich plasma (PRP) augmentation does not result in more favourable outcomes in arthroscopic meniscal repair: a meta-analysis.Journal of orthopaedics and traumatology : official journal of the Italian Society of Orthopaedics and Traumatology · 2022
    Pooled it
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  18. The Role of miRNA in Endometriosis-Related Infertility-An Update.International journal of molecular sciences · 2025
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2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Lusine AghajanovaDepartment of Obstetrics, Gynecology and Reproductive Sciences, Center for Reproductive Sciences, and Center for Reproductive Health, University of California San Francisco, 550 16th Street, 7th Floor, Box 0132, San Francisco, CA, 94158, USA. lusine.aghajanova@ucsf.edu.
Sahar HoushdaranDepartment of Obstetrics, Gynecology and Reproductive Sciences, Center for Reproductive Sciences, and Center for Reproductive Health, University of California San Francisco, 550 16th Street, 7th Floor, Box 0132, San Francisco, CA, 94158, USA.
Shaina BalayanDepartment of Obstetrics, Gynecology and Reproductive Sciences, Center for Reproductive Sciences, and Center for Reproductive Health, University of California San Francisco, 550 16th Street, 7th Floor, Box 0132, San Francisco, CA, 94158, USA.
Evelina ManvelyanDepartment of Obstetrics, Gynecology and Reproductive Sciences, Center for Reproductive Sciences, and Center for Reproductive Health, University of California San Francisco, 550 16th Street, 7th Floor, Box 0132, San Francisco, CA, 94158, USA.
Juan C IrwinDepartment of Obstetrics, Gynecology and Reproductive Sciences, Center for Reproductive Sciences, and Center for Reproductive Health, University of California San Francisco, 550 16th Street, 7th Floor, Box 0132, San Francisco, CA, 94158, USA.
Heather G HuddlestonDepartment of Obstetrics, Gynecology and Reproductive Sciences, Center for Reproductive Sciences, and Center for Reproductive Health, University of California San Francisco, 550 16th Street, 7th Floor, Box 0132, San Francisco, CA, 94158, USA.
Linda C GiudiceDepartment of Obstetrics, Gynecology and Reproductive Sciences, Center for Reproductive Sciences, and Center for Reproductive Health, University of California San Francisco, 550 16th Street, 7th Floor, Box 0132, San Francisco, CA, 94158, USA.
University of California, San Francisco · USReproductive Science Center · US

Funding

Project 4: Human Endometrial Programming for Successful ImplantationP50HD055764 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GIUDICE, LINDA C · 2014 to 2022
$16.4M
Trophoblast Differentiation in In Vivo and In Vitro Fertilized EmbryosU54HD055764 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SONG, JUN S · 2007 to 2013
$11.5M
CRR LIGAND ASSAY AND ANALYSIS CORER24HD102061 · NICHD · UNIVERSITY OF VIRGINIA · PI HAISENLEDER, DANIEL J. · 2019 to 2023
$2.2M
Eunice Kennedy Shriver National Institute of Child Health and Human Development P50HD055764NICHD NIH HHS P50 HD055764NICHD NIH HHS R24 HD102061NICHD NIH HHS U54 HD055764
6 · The paper itself

Abstract

purposeThe study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endometrial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring.

methodsHuman endometrial stromal fibroblasts (eSF), endometrial mesenchymal stem cells (eMSC), bone marrow-derived mesenchymal stem cells (BM-MSC), and Ishikawa endometrial adenocarcinoma cells (IC) were cultured with/without 5% activated (a) PRP, non-activated (na) PRP, aPPP (platelet-poor-plasma), and naPPP. Treatment effects were evaluated with cell proliferation (WST-1), wound healing, and chemotaxis Transwell migration assays. Mesenchymal-to-epithelial transition (MET) was evaluated by cytokeratin and vimentin expression. Differential gene expression of various markers was analyzed by multiplex Q-PCR.

resultsActivated PRP enhanced migration of all cell types, compared to naPRP, aPPP, naPPP, and vehicle controls, in a time-dependent manner (p < 0.05). The WST-1 assay showed increased stromal and mesenchymal cell proliferation by aPRP vs. naPRP, aPPP, and naPPP (p < 0.05), while IC proliferation was enhanced by aPRP and aPPP (p < 0.05). There was no evidence of MET. Expressions of MMP1, MMP3, MMP7, and MMP26 were increased by aPRP (p < 0.05) in eMSC and eSF. Transcripts for inflammation markers/chemokines were upregulated by aPRP vs. aPPP (p < 0.05) in eMSC and eSF. No difference in estrogen or progesterone receptor mRNAs was observed.

conclusionsThis is the first study evaluating the effect of PRP on different human endometrial cells involved in tissue regeneration. These data provide an initial ex vivo proof of principle for autologous PRP to promote endometrial regeneration in clinical situations with compromised endometrial growth and scarring.

Indexed as

Platelet-Rich PlasmaAgedCell MovementCell ProliferationCells, CulturedEndometriumEpithelial-Mesenchymal TransitionFemaleFibroblastsGene Expression RegulationHumansMaleMesenchymal Stem CellsRegenerationEndometriumPlatelet-rich plasmaProliferationRegenerationStem cells

Identifiers

PMID29404863
PMCPMC5984879
OpenAlexW2786915841

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.