Evidence map›Paper›PMID 29404209›Full record

ArticlePeerJ2018

Development of an RNA interference (RNAi) gene knockdown protocol in the anaerobic gut fungus

Shelby S Calkins, Nicole C Elledge, Katherine E Mueller, Stephen M Marek, M B Couger, Mostafa S Elshahed, Noha H Youssef

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Liver Expression ofAnimals : an open access journal from MDPI · 2020
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Shelby S CalkinsDepartment of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK, USA.
Nicole C ElledgeDepartment of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK, USA.
Katherine E MuellerDepartment of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK, USA.
Stephen M MarekDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, USA.
M B CougerHigh Performance Computing Center, Oklahoma State University, Stillwater, OK, USA.
Mostafa S ElshahedDepartment of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK, USA.
Noha H YoussefDepartment of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK, USA.
Oklahoma State University · USTexas A&M University – Corpus Christi · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Members of the anaerobic gut fungi (AGF) reside in rumen, hindgut, and feces of ruminant and non-ruminant herbivorous mammals and reptilian herbivores. No protocols for gene insertion, deletion, silencing, or mutation are currently available for the AGF, rendering gene-targeted molecular biological manipulations unfeasible. Here, we developed and optimized an RNA interference (RNAi)-based protocol for targeted gene silencing in the anaerobic gut fungus

Indexed as

Anaerobic gut fungiD-lactate dehydrogenasePecoramyces ruminantiumRNA interference

Identifiers

PMID29404209
PMCPMC5796279
OpenAlexW2745882352

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.