Evidence map›Paper›PMID 29403209›Full record

ArticleJournal of laboratory physicians

Development of a dry-reagent mix-based polymerase chain reaction as a novel tool for the identification of

Raghavendra D Kulkarni, Mukti Nath Mishra, Jeevanandam Mohanraj, Arun Chandrasekhar, G S Ajantha, Sheetal Kulkani, Shama Bhat

Open access · goldAbstract read
In one paragraph

Article in Journal of laboratory physicians. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Raghavendra D KulkarniDepartment of Microbiology, SDM College of Medical Sciences and Hospital, Dharwad, India.
Mukti Nath MishraDepartment of Molecular Diagnostics, Bhat Biotech India Pvt. Ltd., Bengaluru, Karnataka, India.
Jeevanandam MohanrajDepartment of Molecular Diagnostics, Bhat Biotech India Pvt. Ltd., Bengaluru, Karnataka, India.
Arun ChandrasekharDepartment of Molecular Diagnostics, Bhat Biotech India Pvt. Ltd., Bengaluru, Karnataka, India.
G S AjanthaDepartment of Microbiology, SDM College of Medical Sciences and Hospital, Dharwad, India.
Sheetal KulkaniDepartment of Microbiology, SDM College of Medical Sciences and Hospital, Dharwad, India.
Shama BhatDepartment of Molecular Diagnostics, Bhat Biotech India Pvt. Ltd., Bengaluru, Karnataka, India.
SDM College of Medical Science and Hospital · INBiotech Park · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNosocomial infections are often caused by multidrug-resistant bacteria and the incidence is increasing. AIM AND

objectiveThe present work was focused on comparing the sensitivity and specificity of dry-reagent PCR mix over conventional wet-reagent PCR mix for identification of MATERIALS AND

methodsConventional wet-reagent mix based and dry-reagent mix based PCR were carried out for the DNA isolated from

resultsThe

conclusionPerformance of dry-reagent PCR mix without the need for DNA extraction and preparation of a PCR mix proved to be more sensitive and reduce the handling error, minimizes the time, manual work, and skilled labor.

Indexed as

AcinetobacterDNAdry-reagent mixinhibitorspolymerase chain reaction

Identifiers

PMID29403209
PMCPMC5784298
OpenAlexW2781573909

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.