ArticleScientific reports2018
Effect of Melatonin on the stability and expression of reference genes in Catharanthus roseus.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Effects of RNA Interference-Mediated Silencing of the Insulin-Like Androgenic Gland Hormone Gene on Growth and Gonad Development in the Swimming Crab (Animals : an open access journal from MDPI · 2026Article
- Enhancing the growth and yield of the common bean cultivar 'Nebraska' under high temperature conditions by combining different magnesium levels with arginine, glycine, and melatonin.BMC plant biology · 2025Article
- Effect of Exogenous Melatonin Supply on Potato Plants Grown In Vitro.Antioxidants (Basel, Switzerland) · 2025Article
- Transcriptome analysis ofFrontiers in plant science · 2025Article
- Review
- The role of melatonin in plant growth and metabolism, and its interplay with nitric oxide and auxin in plants under different types of abiotic stress.Frontiers in plant science · 2023Review
- Article
- Selection of Suitable Reference Genes for RT-qPCR Gene Expression Analysis in Siberian Wild Rye (Genes · 2019Article
- Exogenous melatonin trigger biomass accumulation and production of stress enzymes during callogenesis in medicinally importantPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Melatonin and Its Effects on Plant Systems.Molecules (Basel, Switzerland) · 2018Review
- Melatonin Treatment Inhibits the Growth ofFrontiers in microbiology · 2018Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of Melatonin in influencing diverse genes in plants has gained momentum in recent years and many reports have employed qRT-PCR for their quantification. Relative quantification of gene expression relies on accurate normalization of qRT-PCR data against a stably-expressing internal reference-gene. Although researchers have been using commonly available reference-genes to assess Melatonin-induced gene expression, but to-date, there have been no attempts to validate the reference-gene stability under Melatonin-supplementation in planta. In this study, we performed stability assessment of common reference-genes under Melatonin-supplementation and abiotic stress in leaves and seedlings of Catharanthus roseus using geNorm, NormFinder, BestKeeper, ΔCt and RefFinder algorithms. Nine candidate reference-genes were tested for stability in C. roseus (FBOX, CACS, TIP, RSP9, EXP, EXPR, SAND, F17M5, ACT) and our study inferred that while EXP and EXPR were the most-stable, F17M5 was the lowest-stable gene in the leaf-fed samples. Among seedlings of C. roseus, F17M5 and TIP were the most, while ACT was the least-stable gene. The suitability of selected stable reference-gene pairs was demonstrated by assessing the transcript levels of the Melatonin-biosynthesis gene SNAT under same conditions. Our study is the first to comprehensively analyze the stability of commonly-used reference-genes under Melatonin-induced conditions in C. roseus.
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