ArticleMedical principles and practice : international journal of the Kuwait University, Health Science Centre2018
Antibacterial Activity, Cytotoxicity, and the Mechanism of Action of Bacteriocin from Bacillus subtilis GAS101.
Article in Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 94 citations in OpenAlex.
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- Effects of probiotic treatment on the intestinal microbial community of Haliotis diversicolor.AMB Express · 2025Article
- Investigating the production and synergistic antibacterial activity of bacteriocin-like substance fromHeliyon · 2025Article
- Mitigating gut dysbiosis induced by biofilm-forming pathogens: therapeutic potential of LAB-derived bacteriocins.Frontiers in microbiology · 2025Article
- Antibiofilm Analysis, Synergistic Potential and Biocompatibility Evaluation of a Bacteriocin fromIndian journal of microbiology · 2024Article
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- Screening of different growth conditions ofOpen life sciences · 2024Article
- Importance of microbial amphiphiles: interaction potential of biosurfactants, amyloids, and other exo-polymeric-substances.World journal of microbiology & biotechnology · 2023Review
- Bacteriocin Production by Bacillus Species: Isolation, Characterization, and Application.Probiotics and antimicrobial proteins · 2022Review
- Antioxidant, Antibacterial, and Anti-diabetic Activity of Green Synthesized Copper Nanoparticles of Cocculus hirsutus (Menispermaceae).Applied biochemistry and biotechnology · 2022Article
- Microbe Related Chemical Signalling and Its Application in Agriculture.International journal of molecular sciences · 2022Review
- Novel Chronic Wound Healing by Anti-biofilm Peptides and Protease.Advanced pharmaceutical bulletin · 2022Review
- Antimicrobial Impacts of Microbial Metabolites on the Preservation of Fish and Fishery Products: A Review with Current Knowledge.Microorganisms · 2022Review
- Article
- Lactic Acid Bacteria as Antimicrobial Agents: Food Safety and Microbial Food Spoilage Prevention.Foods (Basel, Switzerland) · 2021Review
- Isolation, characterization, and molecular identification of soil bacteria showing antibacterial activity against human pathogenic bacteria.Journal, genetic engineering & biotechnology · 2021Article
- Bacteriocinogenic Potential of Bacillus amyloliquefaciens Isolated from Kimchi, a Traditional Korean Fermented Cabbage.Probiotics and antimicrobial proteins · 2021Article
- Experimental elucidation of an antimycobacterial bacteriocin produced by ethnomedicinal plant-derived Bacillus subtilis (MK733983).Archives of microbiology · 2021Article
- Bacillaene Mediates the Inhibitory Effect of Bacillus subtilis on Campylobacter jejuni Biofilms.Applied and environmental microbiology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe aim of this study was to purify and characterize bacteriocin from the soil isolate Bacillus subtilis GAS101, and to determine its antimicrobial as well as antibiofilm potential. The purified bacteriocin was further analyzed and evaluated for mammalian cell cytotoxicity and the possible mode of action. MATERIAL AND
methodsBacteriocin from B. subtilis GAS101 (an animal husbandry soil isolate) was partially purified and checked for antimicrobial and antibiofilm activity against gram-positive and gram-negative bacteria. The molecular weight of bacteriocin was determined using tricine SDS-PAGE gel. The stability of bacteriocin was investigated at various temperatures and pH levels, and its sensitivity towards 8 enzymes and 6 chemicals was determined. Cytotoxicity analysis was performed on a Vero cell line by a tetrazolium dye-based assay. Scanning electron microscopy (SEM) of bacteriocin-treated bacteria was carried out to determine the possible mode of action.
resultsBacteriocin from B. subtilis GAS101 was a potential inhibitor of both the indicator organisms (Staphylococcus epidermidis and Escherichia coli), and had a molecular weight of approximately 6.5 kDa. An in situ gel assay showed a zone of inhibition corresponding to the estimated protein band size. Bacteriocin was stable and showed antibacterial activity in broad ranges of temperature (30-121°C) and pH (2-12). It was sensitive to 4 proteolytic enzymes, which indicated its proteinaceous nature. Bacteriocin showed > 70% cell viability on the mammalian Vero cell line. SEM depicted that the bacteriocin was able to disrupt the bacterial cell membrane as its probable mode of action.
conclusionThermostable and pH-tolerant bacteriocin from B. subtilis GAS101, of about 6.5 kDa, showed broad-spectrum antimicrobial and antibiofilm activity.
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