ArticleeLife2018
Spatially resolved RNA-sequencing of the embryonic heart identifies a role for Wnt/β-catenin signaling in autonomic control of heart rate.
Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 58 citations in OpenAlex.
- Genetic interrogation reveals mechanisms regulating nexus glia development and shaping heart physiology.Developmental cell · 2026Article
- Regionalized regulation of actomyosin organization influences cardiomyocyte cell shape changes during chamber curvature formation.Nature communications · 2026Article
- Hedgehog and Bmp signaling pathways play opposing roles during establishment of the cardiac inflow tract in zebrafish.Development (Cambridge, England) · 2026Article
- The pericardium forms as a distinct structure during heart formation.Nature communications · 2025Article
- tomoseqr: A Bioconductor package for spatial reconstruction and visualization of 3D gene expression patterns based on RNA tomography.PloS one · 2025Article
- Spns1-dependent endocardial lysosomal function drives valve morphogenesis through Notch1-signaling.iScience · 2024Article
- A Foxf1-Wnt-Nr2f1 cascade promotes atrial cardiomyocyte differentiation in zebrafish.PLoS genetics · 2024Article
- Distinct mechanisms regulate ventricular and atrial chamber wall formation.Nature communications · 2024Article
- scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular players in heart rhythm regulation.iScience · 2024Article
- Unraveling the stereoscopic gene transcriptional landscape of zebrafish using FishSED, a fish spatial expression database with multispecies scalability.Science China. Life sciences · 2024Article
- Development of the Cardiac Conduction System.Advances in experimental medicine and biology · 2024Article
- Single cell RNA-sequencing and RNA-tomography of the avian embryo extending body axis.Frontiers in cell and developmental biology · 2024Article
- Advances and Prospects in Understanding Vertebrate Cardiac Conduction System, Pacemaker Cell, and Cardiac Muscle Development: Toward Novel Biological Therapies.Muscles (Basel, Switzerland) · 2023Review
- Single-cell profiling of the developing embryonic heart in Drosophila.Development (Cambridge, England) · 2023Article
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- The opportunities and challenges of usingFrontiers in physiology · 2023Article
- Stx4 is required to regulate cardiomyocyte CaHGG advances · 2022Article
- Fluid mechanics of the zebrafish embryonic heart trabeculation.PLoS computational biology · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Development of specialized cells and structures in the heart is regulated by spatially -restricted molecular pathways. Disruptions in these pathways can cause severe congenital cardiac malformations or functional defects. To better understand these pathways and how they regulate cardiac development we used tomo-seq, combining high-throughput RNA-sequencing with tissue-sectioning, to establish a genome-wide expression dataset with high spatial resolution for the developing zebrafish heart. Analysis of the dataset revealed over 1100 genes differentially expressed in sub-compartments. Pacemaker cells in the sinoatrial region induce heart contractions, but little is known about the mechanisms underlying their development. Using our transcriptome map, we identified spatially restricted Wnt/β-catenin signaling activity in pacemaker cells, which was controlled by Islet-1 activity. Moreover, Wnt/β-catenin signaling controls heart rate by regulating pacemaker cellular response to parasympathetic stimuli. Thus, this high-resolution transcriptome map incorporating all cell types in the embryonic heart can expose spatially restricted molecular pathways critical for specific cardiac functions.
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