ArticleJournal of immunological methods2018
Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens.
Article in Journal of immunological methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 26 citations in OpenAlex.
- Effect of controlled human Plasmodium falciparum infection on B cell subsets in individuals with different levels of malaria immunity.Medical microbiology and immunology · 2025Trial
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- Differential Patterns of IgG Subclass Responses toFrontiers in immunology · 2019Trial
- Baseline exposure, antibody subclass, and hepatitis B response differentially affect malaria protective immunity following RTS,S/AS01E vaccination in African children.BMC medicine · 2018Trial
- Helminth-infected Mozambican children with malaria have increased anaemia, cytokines and helminth-specific antibodies.PLoS neglected tropical diseases · 2026Article
- Helminth coinfections mitigate clinical, parasitological, and immune outcomes in Mozambican children with malaria.BMC medicine · 2026Article
- Systems biology of malaria explored with nonhuman primates.Malaria journal · 2022Review
- Plasmodium falciparum and Helminth Coinfections Increase IgE and Parasite-Specific IgG Responses.Microbiology spectrum · 2021Article
- Highly Sensitive and Specific Multiplex Antibody Assays To Quantify Immunoglobulins M, A, and G against SARS-CoV-2 Antigens.Journal of clinical microbiology · 2021Article
- Reduced Placental Transfer of Antibodies Against a Wide Range of Microbial and Vaccine Antigens in HIV-Infected Women in Mozambique.Frontiers in immunology · 2021Article
- Framework for Characterizing Longitudinal Antibody Response in Children AfterFrontiers in immunology · 2021Article
- Seroprevalence of antibodies against SARS-CoV-2 among health care workers in a large Spanish reference hospital.Nature communications · 2020Article
- Antibody responses to the RTS,S/AS01NPJ vaccines · 2020Article
- A high-throughput and ultrasensitive identification methodology for unauthorized GMP component based on suspension array and logical calculator.Scientific reports · 2019Article
- Antibody responses to α-Gal in African children vary with age and site and are associated with malaria protection.Scientific reports · 2018Article
- Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgGMalaria journal · 2018Article
- Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundQuantitative suspension arrays are powerful immunoassays to measure antibodies against multiple antigens in large numbers of samples in a short time and using few microliters. To identify antigen targets of immunity for vaccine development against complex microbes like Plasmodium falciparum, such technology allows the characterization of the magnitude and antigenic specificity of Ig isotypes and subclasses that are important for functional responses. However, standardized assays are not widely available.
methodsWe developed six quantitative suspension array assays to measure IgG1, IgG2, IgG3, IgG4, IgM and IgE specific to multiple P. falciparum antigens. Secondary and tertiary antibodies, as well as human purified antibodies for standard curves, were tested among several commercially available sources. Positive and negative controls included plasmas from malaria hyper-immune African adults and from malaria-naïve European adults, respectively. Reagents were selected and optimal antibody and test sample dilutions established according to sensitivity, specificity and performance of the standard curves. The variability between replicates and plates was assessed with 30 test samples and controls.
resultsAssays were able to detect P. falciparum antigen-specific antibodies for all isotypes and subclasses in samples from malaria-exposed individuals, with low background signal in blank wells. Levels detected in malaria-naïve individuals were overall low except for IgM. For the IgG2 and IgE assays, a triple sandwich was required for sensitivity. Standard curves with 5-parameter logistic fit were successfully obtained in all assays. The coefficients of variation for measurements performed in different days were all <30%, and <5% when comparing duplicates from the same plate.
conclusionThe isotype/subclass assays developed here were sensitive, specific, reproducible and of adequate quantification dynamic range. They allow performing detailed immuno-profiling to large panels of P. falciparum antigens to address naturally- and vaccine-induced Ig responses and elucidate correlates of malaria protection, and could also be applied to other antigenic panels.
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