ArticleJournal of industrial microbiology & biotechnology2018
High-level recombinant production of squalene using selected Saccharomyces cerevisiae strains.
Article in Journal of industrial microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 67 citations in OpenAlex.
- Multi-level metabolic engineering for squalene production: from pathway optimization to systematic regulation.World journal of microbiology & biotechnology · 2026Review
- Metabolic Engineering of Yeasts for the Production of the Triterpene Squalene: Current Status and Future Prospective.Microorganisms · 2025Review
- Matrix regulation: a plug-and-tune method for combinatorial regulation in Saccharomyces cerevisiae.Nature communications · 2025Article
- Functional insights from recombinant production of bacterial proteases in Saccharomyces cerevisiae.Microbial cell factories · 2025Article
- Multi-omics analysis ofFrontiers in microbiology · 2025Article
- CRISPR-Mediated rDNA Integration and Fluorescence Screening for Pathway Optimization inChem & bio engineering · 2024Article
- Microbial Squalene: A Sustainable Alternative for the Cosmetics and Pharmaceutical Industry - A Review.Engineering in life sciences · 2024Review
- Enhanced oxidative stability of deuterated squalene: the impact of labelling extent on chemical resilience.RSC advances · 2024Article
- Engineering peroxisomal biosynthetic pathways for maximization of triterpene production inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi.Nature communications · 2023Article
- Metabolic recycling of storage lipids promotes squalene biosynthesis in yeast.Biotechnology for biofuels and bioproducts · 2022Article
- Chain flexibility of medicinal lipids determines their selective partitioning into lipid droplets.Nature communications · 2022Article
- Recent advances in the microbial production of squalene.World journal of microbiology & biotechnology · 2022Review
- Fine-tuning the expression of pathway gene in yeast using a regulatory library formed by fusing a synthetic minimal promoter with different Kozak variants.Microbial cell factories · 2021Article
- Production of Squalene inJournal of agricultural and food chemistry · 2020Article
- Article
- Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities.Frontiers in bioengineering and biotechnology · 2019Review
- Corrigendum: Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities.Frontiers in bioengineering and biotechnology · 2019Article
- Whole-cell (+)-ambrein production in the yeastMetabolic engineering communications · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
For recombinant production of squalene, which is a triterpenoid compound with increasing industrial applications, in microorganisms generally recognized as safe, we screened Saccharomyces cerevisiae strains to determine their suitability. A strong strain dependence was observed in squalene productivity among Saccharomyces cerevisiae strains upon overexpression of genes important for isoprenoid biosynthesis. In particular, a high level of squalene production (400 ± 45 mg/L) was obtained in shake flasks with the Y2805 strain overexpressing genes encoding a bacterial farnesyl diphosphate synthase (ispA) and a truncated form of hydroxyl-3-methylglutaryl-CoA reductase (tHMG1). Partial inhibition of squalene epoxidase by terbinafine further increased squalene production by up to 1.9-fold (756 ± 36 mg/L). Furthermore, squalene production of 2011 ± 75 or 1026 ± 37 mg/L was obtained from 5-L fed-batch fermentations in the presence or absence of terbinafine supplementation, respectively. These results suggest that the Y2805 strain has potential as a new alternative source of squalene production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.